- Clone
- TDP2H4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TAR DNA-binding protein-43, TDP 43, TARDBP, ALS10
- Isotype
- Rat IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TDP2H4_Biotin_TDP43_Antibody_1_081318 TDP2H4_Biotin_TDP43_Antibody_1_081318](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TDP2H4_Biotin_TDP43_Antibody_1_081318.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of Biotin anti-TDP43 antibody (clone TDP2H4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain nuclear lysate; Lane 3: 20 µg of mouse brain nuclear lysate; Lane 4: 20 µg of rat brain nuclear lysate. The blot was incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system. -
IHC staining of Biotin anti-TDP43 antibody (clone TDP2H4) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit were used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of Biotin anti-TDP43 antibody (clone TDP2H4) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit were used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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808303 | 25 µg | 171 CHF | ||||
808304 | 100 µg | 393 CHF |
TDP43, also known as TARDBP, was originally identified as a protein that binds to the transactivation response (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. TDP43 contains two copies of a ~90 amino acid RNA recognition motif (RRM) found in many proteins, which bind single stranded RNA. Recent research has shown that hyperphosphorylated, ubiquitinated, and N-terminally truncated TDP43 is the pathological hallmark lesion in most familial and sporadic forms of FTLD-U and ALS (1).
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
Expected Staining Pattern: Nuclear -
Application References
(PubMed link indicates BioLegend citation) -
- Neumann M. 2009. Int. J. Mol. Sci. 10.3390/ijms10010232.
- RRID
-
AB_2750118 (BioLegend Cat. No. 808303)
AB_2750119 (BioLegend Cat. No. 808304)
Antigen Details
- Structure
- TDP43 is a 414 amino acid protein with an apparent molecular mass of 45 kD.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- TDP43
- Gene ID
- 23435 View all products for this Gene ID 230908 View all products for this Gene ID 100174676 View all products for this Gene ID
- UniProt
- View information about TDP43 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All TDP43 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TDP43 | TDP2H4 | IHC-P,WB,Direct ELISA |
Biotin anti-TDP43 | TDP2H4 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-TDP43
IHC staining of anti-TDP43 antibody (clone TDP2H4) on formal... Western blot of anti-TDP43 antibody (clone TDP2H4). Lane 1: ... Western blot of anti-TDP43 antibody (clone TDP2H4). Lane 1: ... Direct ELISA of purified anti-TDP43 antibody (clone TDP2H4) ... -
Biotin anti-TDP43
Western blot of Biotin anti-TDP43 antibody (clone TDP2H4). L... IHC staining of Biotin anti-TDP43 antibody (clone TDP2H4) on... IHC staining of Biotin anti-TDP43 antibody (clone TDP2H4) on...
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