Biotinylated Recombinant Human Nectin3-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Nectin3, Nectin-3, PVRL3, PVRR3, CD113, CD113 antigen, nectin 3, poliovirus receptor-related 3, poliovirus receptor-related protein3, PPR3, PRR3CD113
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Product Citations
publications
Biotinylated_Recombinant-Human-Nectin3_1_CF
When recombinant human Nectin-1 is immobilized at 2 µg/mL, Biotinylated Recombinant Human Nectin-3 binds in a dose-dependent manner. The ED50 for this effect is 1.4 – 8.4 ng/mL.
  • Biotinylated_Recombinant-Human-Nectin3_1_CF
    When recombinant human Nectin-1 is immobilized at 2 µg/mL, Biotinylated Recombinant Human Nectin-3 binds in a dose-dependent manner. The ED50 for this effect is 1.4 – 8.4 ng/mL.
  • Biotinylated_Recombinant-Human-Nectin3_2_CF
    Stability Testing for Biotinylated Recombinant Human Nectin-3. Biotinylated Recombinant Human Nectin-3 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were evaluated in a binding assay with immobilized recombinant human Nectin-1 at 2 µg/mL and different concentration of Biotinylated Recombinant Human Nectin-3 that binds in a dose-dependent manner. The ED50 for this effect is 1.4 – 8.4 ng/mL.
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568204 25 µg 320 CHF
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568206 100 µg 910 CHF
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Description

Nectin cell adhesion molecule-3 (Nectin-3), also known as Poliovirus receptor-related 3 (PVRL3) and CD113, is a member of the nectin family and type I transmembrane glycoprotein. Nectin-3 belongs to the family of immunoglobulin (Ig)-like cellular adhesion molecules involved in Ca2+-independent cellular adhesion in several tissues during the development. Nectin-3 is abundantly expressed in testis, placenta, lung, liver, kidney, brain, and heart. Nectin-3 has three splicing variants, nectin-3α, -3β, and -3γ. Nectin-3 is highly expressed in epithelial cancer cells of human lung adenocarcinoma and an important player in cellular invasion by upregulating the expression of matrix metalloproteinases (MMP)-2 and -9. Nectin-3 has also been shown to trans-interact with nectin-1, thus plays a key role in the formation of synapses in the brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Biotinylated recombinant human Nectin-3, amino acid Gly58-Asp400 (Accession No. Q9NQS3) was expressed in 293E cells. The C-terminal includes human IgG1-Fc-Avi tag.
Molecular Mass
The 603 amino acid recombinant protein has a predicted molecular mass of approximately 66.7 kD. The DTT-reduced and non-reduced proteins migrates at approximately 70 - 90 kD and 140 - 200 kD by SDS-PAGE, respectively.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH7.2.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Immobilized recombinant human Nectin-1 binds to Biotinylated Recombinant Human Nectin-3 in a dose-dependent manner. The ED50 for this effect is 1.4 – 8.4 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Epithelial cells, granule cells, APC, cancer cells, T-cells, and neuronal cells

Function
Adhesion, Ocular development involving the ciliary body
Interaction
T-lymphocytes, NK cell, epithelial cells
Ligand/Receptor
Nectin-1, -2, and -3, TIGIT, and CD155
Bioactivity
Measured by its ability to bind recombinant human Nectin-1
Cell Type
Antigen-presenting cells, Endothelial cells, Epithelial cells, Neurons, NK cells, T cells
Biology Area
Adaptive Immunity, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, Innate Immune Signaling
Antigen References
  1. Takai, Y. and H. Nakanishi. 2003. J Cell Sci. 116:17-27.
  2. Takai Y, et al. 2008. Annual Review of Cell and Developmental Biology. 24:309-42.
  3. De Agustín-Durán D, et al. 2021. Cells. 10:118.
  4. Satoh-Horikawa K, et al. 2000. J Biol Chem. 275:10291-9.
  5. Inagaki M, et al. 2005. Development. 132:1525-37.
  6. Inagaki M, et al. 2006. Genes Cells. 11:1125-32.
Gene ID
25945 View all products for this Gene ID
UniProt
View information about Nectin3 on UniProt.org
Go To Top Version: 1    Revision Date: 02.02.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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