- Clone
- 3E12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NRP-1, VEGFR165 receptor
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3E12_BV421_CD304_Antibody_FC_1_080213.jpg 3E12_BV421_CD304_Antibody_FC_1_080213.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3E12_BV421_CD304_Antibody_FC_1_080213.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD4 APC and CD304 (clone 3E12) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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145209 | 125 µL | 198 CHF |
CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Extracellular region of mouse CD304
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
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AB_2562358 (BioLegend Cat. No. 145209)
Antigen Details
- Structure
- Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
- Distribution
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Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons
- Function
- Angiogenesis, axon guidance, modulation of the immune response
- Ligand/Receptor
- VEGF165, semaphorin-3A
- Cell Type
- Dendritic cells, Endothelial cells, Neurons, NKT cells, Tregs
- Biology Area
- Angiogenesis, Cancer Biomarkers, Cardiovascular Biology, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
- Molecular Family
- Adaptor Proteins, Adhesion Molecules, CD Molecules
- Antigen References
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1. Yadav M, et al. 2012. J. Exp. Med. 209:1713.
2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723.
3. Hansen W, et al. 2012. J. Exp. Med. 209:2001.
4. Milpied P, et al. 2011. Blood 118:2993. - Gene ID
- 18186 View all products for this Gene ID
- UniProt
- View information about CD304 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD304 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
PE anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
APC anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
PerCP/Cyanine5.5 anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
Brilliant Violet 421™ anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
PE/Cyanine7 anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
Biotin anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
TotalSeq™-A0552 anti-mouse CD304 (Neuropilin-1) | 3E12 | PG |
PE/Dazzle™ 594 anti-mouse CD304 (Neuropilin-1) | 3E12 | FC |
TotalSeq™-B0552 anti-mouse CD304 (Neuropilin-1) | 3E12 | PG |
TotalSeq™-C0552 anti-mouse CD304 (Neuropilin-1) | 3E12 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with purified CD304 (... -
PE anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
APC anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 PE and CD304... -
PerCP/Cyanine5.5 anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 FITC and CD3... -
Brilliant Violet 421™ anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
PE/Cyanine7 anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
Biotin anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
TotalSeq™-A0552 anti-mouse CD304 (Neuropilin-1)
-
PE/Dazzle™ 594 anti-mouse CD304 (Neuropilin-1)
C57BL/6 mouse splenocytes were stained with CD4 APC and CD30... -
TotalSeq™-B0552 anti-mouse CD304 (Neuropilin-1)
-
TotalSeq™-C0552 anti-mouse CD304 (Neuropilin-1)
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