- Clone
- 3/23 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Bp50, TNFRSF5
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![323_BV421_CD40_Antibody_071723 323_BV421_CD40_Antibody_071723](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/323_BV421_CD40_Antibody_071723.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse CD19 PE/Cyanine7 and anti-mouse CD40 (clone 3/23) Brilliant Violet 421™ (left) or rat IgG2a, к Brilliant Violet 421™ isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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124641 | 50 µg | 250 CHF |
CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD40 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 &124628) with a low endotoxin limit (Endotoxin < 0.01 EU/µg).
-
Application References
(PubMed link indicates BioLegend citation) -
- Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
- Bourgeois C, et al. 2002. Science 297:2060.
- RRID
-
AB_3068197 (BioLegend Cat. No. 124641)
Antigen Details
- Structure
- 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
- Distribution
-
Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.
- Function
- Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
- Ligand/Receptor
- CD154 on T cells
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.
- Gene ID
- 21939 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD40 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD40 | 3/23 | FC,IHC-F |
Biotin anti-mouse CD40 | 3/23 | FC |
FITC anti-mouse CD40 | 3/23 | FC |
PE anti-mouse CD40 | 3/23 | FC |
APC anti-mouse CD40 | 3/23 | FC |
Alexa Fluor® 647 anti-mouse CD40 | 3/23 | FC |
PE/Cyanine5 anti-mouse CD40 | 3/23 | FC |
PE/Cyanine7 anti-mouse CD40 | 3/23 | FC |
Ultra-LEAF™ Purified anti-mouse CD40 | 3/23 | FC |
PerCP/Cyanine5.5 anti-mouse CD40 | 3/23 | FC |
Pacific Blue™ anti-mouse CD40 | 3/23 | FC |
PE/Dazzle™ 594 anti-mouse CD40 | 3/23 | FC |
APC/Fire™ 750 anti-mouse CD40 | 3/23 | FC |
TotalSeq™-C0903 anti-mouse CD40 | 3/23 | PG |
TotalSeq™-A0903 anti-mouse CD40 | 3/23 | PG |
APC/Cyanine7 anti-mouse CD40 | 3/23 | FC |
TotalSeq™-B0903 anti-mouse CD40 Antibody | 3/23 | PG |
Brilliant Violet 421™ anti-mouse CD40 | 3/23 | FC |
Brilliant Violet 650™ anti-mouse CD40 | 3/23 | FC |
Brilliant Violet 785™ anti-mouse CD40 | 3/23 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD40
BALB/c mouse splenocytes stained with purified 3/23, followe... Balb/C mouse frozen spleen tissue slices were fixed with 4% ... -
Biotin anti-mouse CD40
BALB/c splenocytes stained with biotinylated 3/23, followed ... -
FITC anti-mouse CD40
BALB/c splenocytes stained with 3/23 FITC -
PE anti-mouse CD40
BALB/c splenocytes stained with 3/23 PE -
APC anti-mouse CD40
BALB/c splenocytes stained with 3/23 APC -
Alexa Fluor® 647 anti-mouse CD40
BALB/c splenocytes stained with 3/23 Alexa Fluor® 647 -
PE/Cyanine5 anti-mouse CD40
C57BL/6 splenocytes stained with 3/23 PE/Cyanine5 -
PE/Cyanine7 anti-mouse CD40
BALB/c splenocytes stained with CD40 (clone 3/23) PE/Cyanine... -
Ultra-LEAF™ Purified anti-mouse CD40
BALB/c mouse splenocytes stained with Ultra-LEAF™ purified C... -
PerCP/Cyanine5.5 anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
Pacific Blue™ anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
PE/Dazzle™ 594 anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
APC/Fire™ 750 anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
TotalSeq™-C0903 anti-mouse CD40
-
TotalSeq™-A0903 anti-mouse CD40
-
APC/Cyanine7 anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
TotalSeq™-B0903 anti-mouse CD40 Antibody
-
Brilliant Violet 421™ anti-mouse CD40
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
Brilliant Violet 650™ anti-mouse CD40
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
Brilliant Violet 785™ anti-mouse CD40
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ...
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