- Clone
- 16A8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- KIA, proliferation-related Ki-67 antigen
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![16A8_BV421_Ki-67_Antibody_FC_111113 16A8_BV421_Ki-67_Antibody_FC_111113](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/16A8_BV421_Ki-67_Antibody_FC_111113.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
652411 | 50 µg | 286 CHF |
The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2562663 (BioLegend Cat. No. 652411)
Antigen Details
- Structure
- 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
- Distribution
-
Nucleus and chromosome
- Function
- Required for cell cycle progression and proliferation
- Interaction
- Interacts with KIF15; binds to MKI67IP through FHA domain
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294. - Gene ID
- 17345 View all products for this Gene ID
- UniProt
- View information about Ki-67 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All Ki-67 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Ki-67 | 16A8 | FC,WB,ICC,IHC-F |
PE anti-mouse Ki-67 | 16A8 | ICFC |
APC anti-mouse Ki-67 | 16A8 | ICFC |
Alexa Fluor® 647 anti-mouse Ki-67 | 16A8 | ICFC |
FITC anti-mouse Ki-67 | 16A8 | ICFC |
Brilliant Violet 421™ anti-mouse Ki-67 | 16A8 | ICFC |
Brilliant Violet 605™ anti-mouse Ki-67 | 16A8 | ICFC |
Alexa Fluor® 488 anti-mouse Ki-67 | 16A8 | ICFC |
Alexa Fluor® 700 anti-mouse Ki-67 | 16A8 | ICFC |
Pacific Blue™ anti-mouse Ki-67 | 16A8 | ICFC |
PerCP/Cyanine5.5 anti-mouse Ki-67 | 16A8 | ICFC |
PE/Cyanine7 anti-mouse Ki-67 | 16A8 | ICFC |
PE/Dazzle™ 594 anti-mouse Ki-67 | 16A8 | ICFC |
APC/Fire™ 750 anti-mouse Ki-67 | 16A8 | ICFC |
Spark NIR™ 685 anti-mouse Ki-67 | 16A8 | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse Ki-67
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were fix... Total cell lysates (15 µg protein) from Raw264.7 and NIH3T3 ... TE‐71 cells were fixed with 1% paraformaldehyde (PFA) for te... C57BL/6 mouse frozen intestine section was fixed with 4% par... -
PE anti-mouse Ki-67
C57BL/6 mouse splenocytes were stimulated for 3 days with Co... -
APC anti-mouse Ki-67
Con A+IL-2 stimulated (3 days) C57BL/6 mouse splenocytes wer... -
Alexa Fluor® 647 anti-mouse Ki-67
Con A-stimulated (3 days) BALB/c mouse splenocytes were fixe... -
FITC anti-mouse Ki-67
Con A-stimulated (3 days) BALB/c mouse splenocytes were fixe... -
Brilliant Violet 421™ anti-mouse Ki-67
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... -
Brilliant Violet 605™ anti-mouse Ki-67
Con A+IL-2-stimulated (3 days) C57BL/6 mouse splenocytes wer... -
Alexa Fluor® 488 anti-mouse Ki-67
Con A-stimulated (3 days) C57BL/6 mouse splenocytes were fix... -
Alexa Fluor® 700 anti-mouse Ki-67
Con A+IL-2 stimulated (3 days) C57BL/6 mouse splenocytes wer... -
Pacific Blue™ anti-mouse Ki-67
Con A-stimulated (three days) C57BL/6 mouse splenocytes were... -
PerCP/Cyanine5.5 anti-mouse Ki-67
Con A+IL-2-stimulated (day 3) C57BL/6 mouse splenocytes were... -
PE/Cyanine7 anti-mouse Ki-67
Con A+IL-2 stimulated (3 days) C57BL/6 mouse splenocytes wer... -
PE/Dazzle™ 594 anti-mouse Ki-67
Con A + IL-2 stimulated (three days) C57BL/6 mouse splenocyt... -
APC/Fire™ 750 anti-mouse Ki-67
Con A-stimulated (2 days) C57BL/6 mouse splenocytes were fix... -
Spark NIR™ 685 anti-mouse Ki-67
Con A+IL-2 stimulated (2 days) C57BL/6 mouse splenocytes wer...
Follow Us