- Clone
- ASL-32 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CEACAM1/5/6, BGP, NCA
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![ASL-32_BV605_CD66ace_Antibody_012819 ASL-32_BV605_CD66ace_Antibody_012819](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/ASL-32_BV605_CD66ace_Antibody_012819.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood granulocytes were stained with CD66a/c/e (clone ASL-32) Brilliant Violet 605™ (closed histogram) or mouse IgG2b, κ Brilliant Violet 605™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
342323 | 25 tests | 193 CHF | ||||
342324 | 100 tests | 402 CHF |
The ASL-32 mAb reacts with an antigen epitope shared by CD66a, c and e. CD66a/c/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Shey MS, et al. 2012. J Immunol Methods. 376:79. PubMed.
- RRID
-
AB_2800909 (BioLegend Cat. No. 342323)
AB_2800910 (BioLegend Cat. No. 342324)
Antigen Details
- Structure
- Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
- Distribution
-
Granulocytes, epithelial cells
- Function
- Homophilic and heterophilic adhesion
- Ligand/Receptor
- CD66a-e, CD62E, galectins
- Cell Type
- Epithelial cells, Granulocytes
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication
- Gene ID
- 634 View all products for this Gene ID
- UniProt
- View information about CD66a CD66c CD66e on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD66a/c/e Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD66a/c/e | ASL-32 | FC,IHC-P |
PE anti-human CD66a/c/e | ASL-32 | FC |
Alexa Fluor® 488 anti-human CD66a/c/e | ASL-32 | FC |
APC anti-human CD66a/c/e | ASL-32 | FC |
PE/Cyanine7 anti-human CD66a/c/e | ASL-32 | FC |
PerCP/Cyanine5.5 anti-human CD66a/c/e | ASL-32 | FC |
Brilliant Violet 421™ anti-human CD66a/c/e | ASL-32 | FC,IHC-P |
Alexa Fluor® 594 anti-human CD66a/c/e | ASL-32 | IHC-P |
Alexa Fluor® 647 anti-human CD66a/c/e | ASL-32 | FC,IHC-P |
TotalSeq™-A0188 anti-human CD66a/c/e | ASL-32 | PG |
APC/Fire™ 750 anti-human CD66a/c/e | ASL-32 | FC |
Brilliant Violet 605™ anti-human CD66a/c/e | ASL-32 | FC |
TotalSeq™-C0188 anti-human CD66a/c/e | ASL-32 | PG |
Spark YG™ 570 anti-human CD66a/c/e | ASL-32 | IHC-P,FC |
TotalSeq™-B0188 anti-human CD66a/c/e | ASL-32 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT29 stained with ... Human paraffin-embedded colon tissue slices were prepared wi... -
PE anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT29 stained with ... -
Alexa Fluor® 488 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 stained with... -
APC anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... Human peripheral blood granulocytes were stained with CD66a/... -
PE/Cyanine7 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... -
PerCP/Cyanine5.5 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... -
Brilliant Violet 421™ anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... Human paraffin-embedded spleen tissue slices were prepared w... -
Alexa Fluor® 594 anti-human CD66a/c/e
Human paraffin-embedded colon tissue slices were prepared wi... -
Alexa Fluor® 647 anti-human CD66a/c/e
Human colorectal adenocarcinoma cell line HT-29 was stained ... Human paraffin-embedded spleen tissue slices were prepared w... -
TotalSeq™-A0188 anti-human CD66a/c/e
-
APC/Fire™ 750 anti-human CD66a/c/e
Human peripheral blood granulocytes were stained with CD66a/... -
Brilliant Violet 605™ anti-human CD66a/c/e
Human peripheral blood granulocytes were stained with CD66a/... -
TotalSeq™-C0188 anti-human CD66a/c/e
-
Spark YG™ 570 anti-human CD66a/c/e
Human paraffin-embedded spleen tissue slices were prepared w... Human paraffin-embedded colon tissue slices were prepared wi... Human colorectal adenocarcinoma cell line HT-29 was stained ... -
TotalSeq™-B0188 anti-human CD66a/c/e
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