- Clone
- L138D7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BLR1, GPCR6, MDR-15
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![L138D7_BV605_CXCR5_Antibody_FC_1_061713 L138D7_BV605_CXCR5_Antibody_FC_1_061713](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L138D7_BV605_CXCR5_Antibody_FC_1_061713.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7) Brilliant Violet 605™ (top) or rat IgG2b, κ Brilliant Violet 605™ isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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145513 | 125 µL | 215 CHF |
CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells. CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow. It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches. Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- mCXCR5-transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.
- Product Citations
-
- RRID
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AB_2562208 (BioLegend Cat. No. 145513)
Antigen Details
- Structure
- 42 kD, seven transmembrane protein
- Distribution
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B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain
- Function
- B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
- Ligand/Receptor
- BLC/CXCL13
- Cell Type
- B cells, T cells, Tfh
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
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1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532.
2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381.
3. Forster R, et al. 1994. Blood 84:830.
4. Forster R, et al. 1996. Cell 87:1037.
5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831. - Gene ID
- 12145 View all products for this Gene ID
- UniProt
- View information about CD185 on UniProt.org
Other Formats
View All CD185 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with purified CXCR5 (... -
PE anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
APC anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
PerCP/Cyanine5.5 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
Biotin anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
Brilliant Violet 421™ anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
Brilliant Violet 605™ anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
PE/Cyanine7 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
Brilliant Violet 650™ anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
FITC anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC a... -
PE/Dazzle™ 594 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
Brilliant Violet 785™ anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 PE an... -
APC/Cyanine7 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
Brilliant Violet 711™ anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC ... -
Alexa Fluor® 647 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 PE an... -
APC/Fire™ 750 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with CD45R/B220 PE an... -
TotalSeq™-A0846 anti-mouse CD185 (CXCR5)
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TotalSeq™-C0846 anti-mouse CD185 (CXCR5)
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TotalSeq™-B0846 anti-mouse CD185 (CXCR5) Antibody
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Brilliant Violet 750™ anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
Spark Red™ 718 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
APC/Fire™ 810 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with anti-mouse/human... -
Spark NIR™ 685 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
PE/Fire™ 640 anti-mouse CD185 (CXCR5)
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R...
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