- Clone
- M1310G05 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M1310G05_BV711_FC_Antibody_071023 M1310G05_BV711_FC_Antibody_071023](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M1310G05_BV711_FC_Antibody_071023.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human IgG Fc (clone M1310G05) Brilliant Violet 711™ (left) or rat IgG2a, к Brilliant Violet 711™ isotype control (right). Cells were costained with anti-human CD19 APC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
410739 | 25 tests | 221 CHF | ||||
410740 | 100 tests | 435 CHF |
IgG Fc is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human Siglec-E-IgG Fc fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
Clone M1310G05 recognizes IgG in the membrane of memory B cells, has a stronger affinity for IgG1 and IgG3 than for IgG2 and IgG4, and does not cross react with IgD, IgE, or IgM.
- RRID
-
AB_3083398 (BioLegend Cat. No. 410739)
AB_3083398 (BioLegend Cat. No. 410740)
Antigen Details
- Structure
- Homodimer formed by the constant region of the IgG heavy chain.
- Ligand/Receptor
- CD16, CD32, and CD64.
- Cell Type
- B cells
- Biology Area
- Immunology
- Antigen References
-
1. Paul, WE. (2003). Fundamental Immunology. Philadelphia, PA: Lippincott, Williams, & Wilkins.
- Gene ID
- 3500 View all products for this Gene ID
- UniProt
- View information about IgG Fc on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All IgG Fc Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human IgG Fc | M1310G05 | FC,ELISA |
Brilliant Violet 421™ anti-human IgG Fc | M1310G05 | FC |
Alexa Fluor® 488 anti-human IgG Fc | M1310G05 | FC |
PE anti-human IgG Fc | M1310G05 | FC |
PerCP/Cyanine5.5 anti-human IgG Fc | M1310G05 | FC |
APC anti-human IgG Fc | M1310G05 | FC |
FITC anti-human IgG Fc | M1310G05 | FC |
Biotin anti-human IgG Fc | M1310G05 | FC |
Brilliant Violet 510™ anti-human IgG Fc | M1310G05 | FC |
Alexa Fluor® 647 anti-human IgG Fc | M1310G05 | FC |
PE/Cyanine7 anti-human IgG Fc | M1310G05 | FC |
APC/Fire™ 750 anti-human IgG Fc | M1310G05 | FC |
TotalSeq™-A0375 anti-human IgG Fc | M1310G05 | PG |
TotalSeq™-C0375 anti-human IgG Fc | M1310G05 | PG |
TotalSeq™-B0375 anti-human IgG Fc | M1310G05 | PG |
APC/Cyanine7 anti-human IgG Fc | M1310G05 | FC |
KIRAVIA Blue 520™ anti-human IgG Fc | M1310G05 | FC |
TotalSeq™-D0375 anti-human IgG Fc | M1310G05 | PG |
PE/Dazzle™ 594 anti-human IgG Fc | M1310G05 | FC |
Brilliant Violet 711™ anti-human IgG Fc | M1310G05 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 421™ anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 AP... -
Alexa Fluor® 488 anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 AP... -
PE anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 AP... -
PerCP/Cyanine5.5 anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 FI... -
APC anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 FI... -
FITC anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 AP... -
Biotin anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 510™ anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 AP... -
Alexa Fluor® 647 anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 P... -
PE/Cyanine7 anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 BV... -
APC/Fire™ 750 anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 BV... -
TotalSeq™-A0375 anti-human IgG Fc
-
TotalSeq™-C0375 anti-human IgG Fc
-
TotalSeq™-B0375 anti-human IgG Fc
-
APC/Cyanine7 anti-human IgG Fc
Human peripheral blood lymphocytes were stained with CD19 FI... -
KIRAVIA Blue 520™ anti-human IgG Fc
Human PBMCs were stained with anti-human IgG Fc (clone M1310... -
TotalSeq™-D0375 anti-human IgG Fc
-
PE/Dazzle™ 594 anti-human IgG Fc
Overnight human peripheral blood lymphocytes were stained wi... -
Brilliant Violet 711™ anti-human IgG Fc
Human peripheral blood lymphocytes were stained with anti-hu...
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