- Clone
- 104 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ly-5.2, LCA
- Isotype
- Mouse (SJL) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![104_BV750_CD45-2_Antibody_011520.png 104_BV750_CD45-2_Antibody_011520.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/104_BV750_CD45-2_Antibody_011520.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD45.2 (clone 104) Brilliant Violet 750™ (filled histogram). SJL/J mouse splenocytes were stained with CD45.2 (clone 104) Brilliant Violet 750™ (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
109857 | 50 µg | 333 CHF |
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.S mouse thymocytes and splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.
- Concentration
- 0.2 mg/mL
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
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Application References
(PubMed link indicates BioLegend citation) -
- Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
- Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
- Suzuki K, et al. 2000. Immunity 13:691. (IHC)
- Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
- Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
- Pascal V, et al. 2007. J. Immunol. 179:1751.
- Burman AC, et al. 2007. Blood 110:1064.
- Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
- Phan TG, et al. 2007. Nature Immunol. 8:992.
- Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
- Wen T, et al. 2013. PNAS. 110:6067. PubMed
- Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
- RRID
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AB_2832376 (BioLegend Cat. No. 109857)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
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All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
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1. Suzuki K, et al. 2000. Immunity 13:691.
- Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45.2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD45.2 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with biotinylated 104, fol... -
FITC anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 FITC -
PE anti-mouse CD45.2
BALB/c (solid line) and SJL (broken line) splenocytes staine... -
Purified anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with purified 104, followe... -
APC anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 APC -
Alexa Fluor® 488 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 488 -
Alexa Fluor® 647 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® 647 -
Pacific Blue™ anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with Pacific Blue™ 1... -
Alexa Fluor® 700 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 Alexa Fluor® ... -
APC/Cyanine7 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
PerCP anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PerCP -
PerCP/Cyanine5.5 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PerCP/Cyanine5.5 -
PE/Cyanine7 anti-mouse CD45.2
C57BL/6 mouse splenocytes stained with 104 PE/Cyanine7 -
Brilliant Violet 421™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 570™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 650™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 510™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 785™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 605™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Purified anti-mouse CD45.2 (Maxpar® Ready)
C57BL/6 mouse bone marrow cells stained with 147Sm-anti-CD45... -
PE/Dazzle™ 594 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Brilliant Violet 711™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Alexa Fluor® 594 anti-mouse CD45.2
BALB/C mouse frozen spleen section was fixed with 4% parafor... -
APC/Fire™ 750 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
TotalSeq™-A0157 anti-mouse CD45.2
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TotalSeq™-B0157 anti-mouse CD45.2
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TotalSeq™-C0157 anti-mouse CD45.2
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Brilliant Violet 750™ anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Spark Blue™ 550 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 10... -
Spark NIR™ 685 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with anti-mouse CD45.... -
Spark UV™ 387 anti-mouse CD45.2
C57BL/6 mouse splenocytes were stained with anti-mouse CD45.... -
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™)
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Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™)
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