- Clone
- QA20A89 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA20A89_FITC_CD294_Antibody_113023 QA20A89_FITC_CD294_Antibody_113023](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA20A89_FITC_CD294_Antibody_113023.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes were stained with anti-human CD294 (CRTH2) recombinant (clone QA20A89) FITC (left) or mouse IgG1, κ FITC isotype control (right), then anti-human CD4 APC and anti-human CCR4 Brilliant Violet 421™. Data shown was gated on the CD4+ population.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
379805 | 25 tests | 100 CHF | ||||
379806 | 100 tests | 225 CHF |
CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
- RRID
-
AB_3097324 (BioLegend Cat. No. 379805)
AB_3097324 (BioLegend Cat. No. 379806)
Antigen Details
- Distribution
-
Effector T helper 2 (Th2) cells, eosinophils, basophils
- Function
- Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
- Interaction
- Heterotrimeric G proteins
- Ligand/Receptor
- Prostaglandin D2
- Cell Type
- Basophils, Eosinophils, Th2
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Luster AD and Tager AM. 2004. Nat Rev Immunol. 4:711-24.
- Monneret G, et al. 2001. Blood. 98:1942-8.
- Xue L, et al. 2009. J Immunol. 182:7580-6.
- Schratl P, et al. 2007. J Immunol. 179:4792-9.
- Gene ID
- 11251 View all products for this Gene ID
- UniProt
- View information about CD294 on UniProt.org
Other Formats
View All CD294 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD294 (CRTH2) Recombinant Antibody | QA20A89 | FC |
APC anti-human CD294 (CRTH2) Recombinant Antibody | QA20A89 | FC |
FITC anti-human CD294 (CRTH2) Recombinant Antibody | QA20A89 | FC |
PE anti-human CD294 (CRTH2) Recombinant Antibody | QA20A89 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD294 (CRTH2) Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD294 (CRTH2) Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD294 (CRTH2) Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD294 (CRTH2) Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us