- Clone
- m2B4 (B6)458.1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- 2B4 B6 Alloantigen
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 splenocytes stained with m2B4(B6)458.1 FITC and NK-1.1 APC
Cat # | Size | Price | Quantity Check Availability | Save | ||
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133503 | 50 µg | 83 CHF | ||||
133504 | 500 µg | 323 CHF |
Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. There are at least two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which is expressed on all hematopoietic cells. It was reported that CD244 interaction with CD48 is essential for IL-2-driven expansion and activation of murine NK cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- m2B4-Fc
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
- Application Notes
-
The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on BALB/c mice.
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Application References
(PubMed link indicates BioLegend citation) -
- Lee KM, et al. 2006. Blood. 107:3181.
- Schatzle JD, et al. 1999. P. Natl Acad Sci USA. 96:3870.
- Stepp SE, et al. 1999. Eur. J. Immunol. 29:2392.
- de la Luz Sierra M, et al. 2010. Blood. 115:3970. PubMed
- Product Citations
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- RRID
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AB_1595624 (BioLegend Cat. No. 133503)
AB_1595642 (BioLegend Cat. No. 133504)
Antigen Details
- Structure
- A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).
- Distribution
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All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes.
- Function
- Mediate non-MHC-restricted cytotoxicity.
- Ligand/Receptor
- CD48
- Cell Type
- Dendritic cells, NK cells, NKT cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Brown MH, et al. 1998. J. Exp. Med. 188:2083.
2. Davis SJ, et al. 1996. Immunol Today. 17:177.
3. Kumaresan PR, et al. 2000. Immunogenetics. 51:306.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809. - Gene ID
- 18106 View all products for this Gene ID
- UniProt
- View information about CD244.2 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All CD244.2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC,IP,IHC,ELISA |
FITC anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC |
Biotin anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC |
PE anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC |
Alexa Fluor® 647 anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC |
PE/Cyanine7 anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC |
PerCP/Cyanine5.5 anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC |
APC/Fire™ 750 anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC |
APC anti-mouse CD244.2 (2B4 B6 Alloantigen) | m2B4 (B6)458.1 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4(B6)458.1 FITC and NK-1... -
FITC anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4(B6)458.1 FITC and NK-1... -
Biotin anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4 (B6) 458.1 biotin (upp... -
PE anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4(B6)458.1 PE (upper pan... -
Alexa Fluor® 647 anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes stained with m2B4(B6)458.1 Alexa Fluor&r... -
PE/Cyanine7 anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes were stained with CD49b FITC and CD244.2... -
PerCP/Cyanine5.5 anti-mouse CD244.2 (2B4 B6 Alloantigen)
Human peripheral blood lymphocytes were stained with CD49b F... -
APC/Fire™ 750 anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes were stained with CD49b PE and CD244.2 ... -
APC anti-mouse CD244.2 (2B4 B6 Alloantigen)
C57BL/6 splenocytes were stained with CD49b PE and CD244.2 (...
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