- Clone
- 9H10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
- Isotype
- Syrian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![9H10_GoInVivo_CD152_Antibody_080916 9H10_GoInVivo_CD152_Antibody_080916](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9H10_GoInVivo_CD152_Antibody_080916.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Anti-mouse CD152 (CTLA-4) inhibits production of IL-2 by Jurkat cells. When Jurkat cells are stimulated with PHA (2.5 µg/ml) and soluble CD80 (B7.1, 500 ng/ml), the addition of soluble mouse CTLA-4 competes with CD80 and inhibits the production of IL-2 in a dose dependent manner (diamonds). When a fixed concentration of CTLA-4 is selected and pre-incubated with increasing concentrations of anti-mouse CTLA-4 (clone 9H10), the production of IL-2 is restored as the antibody blocks the activity of CTLA-4, but not the isotype control (clone SHG-1).
CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation. The 9H10 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- Mouse CTLA-4-human IgG1 fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
- Preparation
- The GoInVivo™ antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
- Application
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ELISA - Quality tested
Block, Costim, FC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment. - Application Notes
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GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.
Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking1-4 of CTLA-4 action by soluble antibody and promotion of in vitro CTLA-4 signalling by immobilized antibody1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No. 405204)). -
Application References
(PubMed link indicates BioLegend citation) -
- Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block, Costim, FC)
- Krummel MF, et al. 1996. Int. Immunol. 8:519. (Block)
- Leach DR, et al. 1996. Science 271:1734. (Block)
- Wagner Jr, DH, et al. 1996. J. Exp. Med. 184:1631. (Block)
- Product Citations
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- RRID
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AB_2616632 (BioLegend Cat. No. 106208)
AB_2616633 (BioLegend Cat. No. 106209)
AB_2616631 (BioLegend Cat. No. 106207)
AB_2616630 (BioLegend Cat. No. 106206)
AB_2616634 (BioLegend Cat. No. 106210)
AB_2616629 (BioLegend Cat. No. 106205)
- Disclaimer
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This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Antigen Details
- Structure
- Ig superfamily, 33 kD
- Distribution
-
Activated T cells
- Function
- Negative regulator of T cell activation
- Ligand/Receptor
- CD80 (B7-1), CD86 (B7-2)
- Cell Type
- T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Allison JP, et al. 1995. Science 270:932.
3. Waterhouse P, et al. 1995. Science 270:985.
4. Linsley PS, et al. 1991. J. Exp. Med. 174:561. - Gene ID
- 12477 View all products for this Gene ID
- UniProt
- View information about CD152 on UniProt.org
Other Formats
View All CD152 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD152 | 9H10 | ELISA,FC,Block,Costim |
GoInVivo™ Purified anti-mouse CD152 | 9H10 | ELISA,FC,Block,Costim |
Ultra-LEAF™ Purified anti-mouse CD152 | 9H10 | ELISA,FC,Block,Costim |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse CD152
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GoInVivo™ Purified anti-mouse CD152
Anti-mouse CD152 (CTLA-4) inhibits production of IL-2 by Jur... -
Ultra-LEAF™ Purified anti-mouse CD152
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