- Clone
- 1D9-M12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GPR77, C5a receptor-like 2, C5a anaphylatoxin chemotactic receptor
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1D9-M12_PE_050409.jpg 1D9-M12_PE_050409.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1D9-M12_PE_050409.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood granulocytes stained with 1D9-M12 PE
Cat # | Size | Price | Quantity Check Availability | Save | ||
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342403 | 25 tests | 183 CHF | ||||
342404 | 100 tests | 360 CHF |
C5L2 (C5a receptor-like 2) is a seven-transmembrane orphan receptor, also known as GPR77, which shares significant homology to C5a receptor (CD88), but it is a decoy receptor without G-protein coupling. C5L2 is co-expressed with C5aR on many kinds of cells, such as neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes. C5L2 binds C5a with similar high affinity as C5aR and relatively higher affinity for C5a-des-Arg than with C5aR.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications include: Western blotting1,3 and blocking2,3. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
Clone 1D9-M12 is known to block C5a binding to C5L2, but does not react with C5a receptor. It has been reported that this clone is not effective in immunohistochemistry of formalin-fixed paraffin-embedded sections1.
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Application References
(PubMed link indicates BioLegend citation) -
- Fonseca MI, et al. 2013. J. Neuroinflammation. 10:25. (WB) PubMed
- Hao J, et al. 2013. PLoS One 8:e66305. (Block)
- Raby AC, et al. 2011. Eur. J. Immunol. 41:2741. (Block, WB)
- Product Citations
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- RRID
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AB_2113253 (BioLegend Cat. No. 342403)
AB_2247831 (BioLegend Cat. No. 342404)
Antigen Details
- Structure
- Seven transmembrane protein, decoy receptor, does not couple to G-proteins.
- Distribution
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Neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes.
- Function
- Negative regulation of C5a function.
- Ligand/Receptor
- C5a, C5a-des-Arg.
- Cell Type
- Astrocytes, Dendritic cells, Macrophages, Mast cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
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1. Scola AM, et al. 2009. Mol. Immunol. 46:1149
2. Johswich K, et al. 2006. J. Bio. Chem. 281:39088
3. Gerad NP, et al. 2005. J. Bio. Chem. 280:39677
4. Okinaga S, et al. 2003. Biochemistry. 42:9406 - Gene ID
- 27202 View all products for this Gene ID
- UniProt
- View information about C5L2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All C5L2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human C5L2 | 1D9-M12 | FC,WB |
PE anti-human C5L2 | 1D9-M12 | FC |
APC anti-human C5L2 | 1D9-M12 | FC |
TotalSeq™-C0572 anti-human C5L2 | 1D9-M12 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human C5L2
Human peripheral blood granulocytes stained with purified 1D... -
PE anti-human C5L2
Human peripheral blood granulocytes stained with 1D9-M12 PE -
APC anti-human C5L2
Human peripheral blood granulocytes were stained with C5L2 (... -
TotalSeq™-C0572 anti-human C5L2
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