- Clone
- B032F6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Epstein Barr virus induced gene 3, IL-27B, IL-35 subunit
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![B032F6_PE_EBI3_Antibody_FC_1_111313 B032F6_PE_EBI3_Antibody_FC_1_111313](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/B032F6_PE_EBI3_Antibody_FC_1_111313.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes were stimulated with IFNγ, LPS, and R848 for 24 hours (in the presence of Brefeldin A). Cells were then fixed, permeabilized, and stained with CD14 APC and EBI3 (clone B032F6) PE (top) or mouse IgG2b, κ PE isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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360903 | 25 tests | 146 CHF | ||||
360904 | 100 tests | 360 CHF |
Epstein-Barr virus induced gene-3, also known as EBI3, is an IL-12 p40-related protein that can heterodimerize with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 diferentiation, increase cytolytic potential of CD8+ T cells, and impact B cell differentiation and Ig production.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified human IL-27
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
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- RRID
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AB_2562879 (BioLegend Cat. No. 360903)
AB_2562880 (BioLegend Cat. No. 360904)
Antigen Details
- Structure
- Hematopoietin receptor family, subunit of IL-27 and IL-35
- Distribution
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B cells, monocytes and dendritic cells
- Function
- Pro- and anti-inflammatory
- Interaction
- IL-27 p28 subunit, IL-35 p35 subunit
- Cell Type
- B cells, Dendritic cells, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Pflanz S, et al. 2002. Immunity. 16: 779.
2. Takeda A, et al. 2003. J. immunol. 170: 4886.
3. Pflanz S, et al. 2004. J. Immunol. 172: 2225.
4. Li MF, et al. 2013. Dev. Comp. Immunol. 41: 514. - Gene ID
- 10148 View all products for this Gene ID
- UniProt
- View information about IL-27 IL-35 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All IL-27/IL-35 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human IL-27/IL-35 EBI3 (monomer, dimer, heterodimer) | B032F6 | ICFC |
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PE anti-human IL-27/IL-35 EBI3 (monomer, dimer, heterodimer)
Human peripheral blood monocytes were stimulated with IFNγ, ...
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