PE anti-mouse MD-1 Antibody

Pricing & Availability
Clone
MD-113 (See other available formats)
Regulatory Status
RUO
Other Names
MD1
Isotype
Rat IgG2b, κ
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Product Citations
publications
MD-113_PE_091207
C57BL/6 mouse splenocytes stained with MD-113 PE
  • MD-113_PE_091207
    C57BL/6 mouse splenocytes stained with MD-113 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
117805 50 µg 214 CHF
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Description

MD-1 is a small 28-kD molecule that is usually found in association with CD180 (RP105), a member of the Toll-like receptor family. MD-1 is primarily expressed by B cells, dendritic cells and monocytes, and promotes the recognition of, and subsequent signaling by, LPS in the innate immune system. The MD-113 antibody is reported to be antagonistic for LPS-induced B cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse RP105/MD-1 transfected NRK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of LPS-induced B cell proliferation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 117808).

Application References

(PubMed link indicates BioLegend citation)
  1. Nagai Y, et al. 2002. Blood 99 :1699.
RRID
AB_2137928 (BioLegend Cat. No. 117805)

Antigen Details

Structure
Acts in conjunction with the toll-like receptor family members, 28 kD
Distribution

B cells, dendritic cells, and monocytes/macrophages

Function
Recognition and signaling in response to LPS
Ligand/Receptor
LPS
Cell Type
B cells, Monocytes, Macrophages, Dendritic cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Nagai Y, et al. 2002. Blood 99 :1699.

Gene ID
17084 View all products for this Gene ID
UniProt
View information about MD-1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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