PE anti-YAP1 Antibody

Pricing & Availability
Clone
W19260A (See other available formats)
Regulatory Status
RUO
Other Names
Yes1 Associated Transcriptional Regulator, YAP65, Yes-Associated Protein YAP65 Homolog, Transcriptional Coactivator YAP1, Yes Associated Protein 1, COB1, YAP2, YAP, YKI
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19260A_PE_YAP1_1_Antibody_102522
HeLa cells (positive control) (filled histogram) and Jurkat cells (negative control) (open histogram) were fixed and permeabilized using True Nuclear™ Transcription Factor Buffer (Cat. No. 424401) and intracellularly stained with PE anti-YAP1 (clone W19260A), or PE rat IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400636).
  • W19260A_PE_YAP1_1_Antibody_102522
    HeLa cells (positive control) (filled histogram) and Jurkat cells (negative control) (open histogram) were fixed and permeabilized using True Nuclear™ Transcription Factor Buffer (Cat. No. 424401) and intracellularly stained with PE anti-YAP1 (clone W19260A), or PE rat IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400636).
See PE spectral data
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603653 25 tests 206 CHF
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603654 100 tests 405 CHF
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Description

Yes-associated protein 1, or YAP1, is a transcriptional co-activator vital to cell proliferation and the regulation of apoptosis. YAP1 does not contain a DNA-binding domain and requires interaction with transcriptional factors, particularly TEA domain (TEAD) proteins, to regulate transcription. YAP1 is continuously shuttled between the nucleus, where it is primarily located when Hippo signaling is inactive, and the cytoplasm, where it is sequestered following phosphorylation of its various serine residues by the LATS1/2-MOB1 complex of the Hippo pathway or by kinases AKT, PRP4K, and CDK1. As a key effector of the Hippo signaling pathway along with its paralog TAZ, YAP1 is essential to the regulation of cell growth, stem cell maintenance, organ size, tissue homeostasis, regeneration, embryonic development, and the preservation of genome integrity following DNA damage. YAP1 also functions in the modulation of aerobic glycolysis and glutaminolysis pathways as well as amino acid metabolism. YAP1 can act as a tumor suppressor, usually through its interaction with p53 family proteins and promotion of apoptosis, but most commonly functions as an oncogene. Its overexpression and deregulation are characteristic of several cancers and it has been implicated in the initiation, proliferation, metastasis, metabolic reprogramming, immune escape, and therapy resistance of cancer cells. As such, YAP1 is a potential therapeutic target and prognostic biomarker.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human YAP1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For ICC, we recommend 4% PFA fixation followed by permeabilization with ice-cold methanol or 0.5% Triton-X. Fixation and permeabilization with only methanol is not recommended. 

RRID
AB_2927898 (BioLegend Cat. No. 603653)
AB_2927898 (BioLegend Cat. No. 603654)

Antigen Details

Structure
YAP1 is a 504 amino acid protein with a predicted molecular weight of 54 kD.
Distribution

Ubiquitous/ Primarily nuclear localization at low density and primarily cytoplasmic localization at high density 

Function
Transcription
Interaction
TAZ, TEAD1/2/3/4, LATS1/2, YES, SRC, SMAD, p73, RUNX2, PAX3, ERBB4, CK1, ZO1/2
Biology Area
Cancer Biomarkers, DNA Repair/Replication, Stem Cells, Transcription Factors
Antigen References
  1. Chen X, et al. 2020. Front Physiol. 11:389.
  2. Isfort I, et al. 2019. Sci Rep. 9:19704.
  3. Raj N and Bam R. 2019. Front. Cell Dev Biol. 7:159.
  4. Szulzewsky F, et al. 2021. Dev Biol. 475:205-221.
Gene ID
10413 View all products for this Gene ID
UniProt
View information about Yap1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All Yap1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-YAP1 W19260A WB,IHC-P,ICC,ICFC
PE anti-YAP1 W19260A ICFC
Go To Top Version: 1    Revision Date: 09.27.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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