- Clone
- 4B10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-box expressed in T cells, T box 21, TBLYM
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![4B10_PECyanine5_Tbet_Antibody_070324 4B10_PECyanine5_Tbet_Antibody_070324](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/4B10_PECyanine5_Tbet_Antibody_070324.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were surfaced stained with anti-human CD3 (clone UCHT1) Brilliant Violet 421™. Cells were then fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-human T-bet (clone 4B10) PE/Cyanine5 (left) or mouse IgG1, κ PE/Cyanine5 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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644841 | 25 tests | 160 CHF | ||||
644842 | 100 tests | 395 CHF |
T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601). -
Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Antigen Details
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
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Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
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1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907. - Gene ID
- 30009 View all products for this Gene ID
- UniProt
- View information about T-bet on UniProt.org
Other Formats
View All T-bet Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-T-bet | 4B10 | ICFC |
Purified anti-T-bet | 4B10 | WB,ICFC,IHC-F,IP |
Alexa Fluor® 647 anti-T-bet | 4B10 | ICFC |
PerCP/Cyanine5.5 anti-T-bet | 4B10 | ICFC |
Pacific Blue™ anti-T-bet | 4B10 | ICFC |
PE anti-T-bet | 4B10 | ICFC |
Brilliant Violet 711™ anti-T-bet | 4B10 | ICFC |
FITC anti-T-bet | 4B10 | ICFC |
Brilliant Violet 421™ anti-T-bet | 4B10 | ICFC |
Brilliant Violet 605™ anti-T-bet | 4B10 | ICFC |
PE/Cyanine7 anti-T-bet | 4B10 | ICFC |
PE/Dazzle™ 594 anti-T-bet | 4B10 | ICFC |
Purified anti-T-bet (Maxpar® Ready) | 4B10 | WB,CyTOF® |
Alexa Fluor® 488 anti-T-bet | 4B10 | ICFC |
Brilliant Violet 785™ anti-T-bet | 4B10 | ICFC |
Alexa Fluor® 594 anti-T-bet | 4B10 | ICFC,ICC |
KIRAVIA Blue 520™ anti-T-bet | 4B10 | ICFC |
PE/Fire™ 810 anti-T-bet | 4B10 | ICFC |
PE/Cyanine5 anti-T-bet | 4B10 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Purified anti-T-bet
Total cell lysate from PBMC (lane 1, 15 µg) and PBMC treated... Human peripheral blood lymphocytes were surface stained with... Total lysates (15µg protein) from Daudi, K562 and NK-92 cell... -
Alexa Fluor® 647 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PerCP/Cyanine5.5 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Pacific Blue™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 711™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
FITC anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 421™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 605™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE/Cyanine7 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE/Dazzle™ 594 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Purified anti-T-bet (Maxpar® Ready)
Human PBMCs were fixed, permeabilized, and stained with 154S... -
Alexa Fluor® 488 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 785™ anti-T-bet
Human peripheral blood lymphocytes were stained with CD3 APC... -
Alexa Fluor® 594 anti-T-bet
NK-92 cells were fixed with 4% paraformaldehyde (PFA) for 15... NK-92 cells were fixed with 4% paraformaldehyde (PFA) for 15... PBMCs were surface stained with Alexa Fluor® 488 anti-CD3 (C... -
KIRAVIA Blue 520™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE/Fire™ 810 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE/Cyanine5 anti-T-bet
Human peripheral blood mononuclear cells were surfaced stain...
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