- Clone
- MHE-18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin E
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MHE-18_PECy7_1_070212 MHE-18_PECy7_1_070212](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MHE-18_PECy7_1_070212.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood leukocytes were stained with CCR3 APC and anti-human IgE (clone MHE-18) PE/Cyanine7 (top) or mouse IgG1, κ PE/Cyanine7 (bottom). Data shown was gated on the lymphocyte population. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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325509 | 25 tests | 159 CHF | ||||
325510 | 100 tests | 347 CHF |
IgE is the seventh immunoglobulin made by B cells in the immune response.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.
- Product Citations
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- RRID
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AB_11218597 (BioLegend Cat. No. 325509)
AB_11219395 (BioLegend Cat. No. 325510)
Antigen Details
- Distribution
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B cells
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 3497 View all products for this Gene ID
- UniProt
- View information about IgE on UniProt.org
Related FAQs
Other Formats
View All IgE Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human IgE | MHE-18 | FC,ELISA,IHC |
Biotin anti-human IgE | MHE-18 | FC,ELISA,IHC |
PE anti-human IgE | MHE-18 | FC |
APC anti-human IgE | MHE-18 | FC |
PE/Cyanine7 anti-human IgE | MHE-18 | FC |
PerCP/Cyanine5.5 anti-human IgE | MHE-18 | FC |
Alexa Fluor® 647 anti-human IgE | MHE-18 | FC |
APC/Fire™ 750 anti-human IgE | MHE-18 | FC |
TotalSeq™-A0868 anti-human IgE | MHE-18 | PG |
APC/Cyanine7 anti-human IgE | MHE-18 | FC |
TotalSeq™-C0868 anti-human IgE | MHE-18 | PG |
TotalSeq™-B0868 anti-human IgE | MHE-18 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IgE
Overnight cultured human peripheral blood mononuclear cells ... -
Biotin anti-human IgE
Overnight cultured human peripheral blood mononuclear cells ... -
PE anti-human IgE
Overnight cultured human peripheral blood mononuclear cells ... -
APC anti-human IgE
Human peripheral blood leukocytes were stained with CCR3 PE ... -
PE/Cyanine7 anti-human IgE
Human peripheral blood leukocytes were stained with CCR3 APC... -
PerCP/Cyanine5.5 anti-human IgE
Human peripheral blood leukocytes were stained with CCR3 APC... -
Alexa Fluor® 647 anti-human IgE
Overnight cultured human peripheral blood mononuclear cells ... -
APC/Fire™ 750 anti-human IgE
Overnight cultured human peripheral blood mononuclear cells ... -
TotalSeq™-A0868 anti-human IgE
-
APC/Cyanine7 anti-human IgE
Overnight cultured human peripheral blood mononuclear cells ... -
TotalSeq™-C0868 anti-human IgE
-
TotalSeq™-B0868 anti-human IgE
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