- Clone
- S18018E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T8, Lyt2, Ly-2
- Isotype
- Rat IgG2b, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S18018E_PE-Cyanine7_CD8a_Antibody_081123 S18018E_PE-Cyanine7_CD8a_Antibody_081123](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S18018E_PE-Cyanine7_CD8a_Antibody_081123.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε APC and anti-mouse CD8a (clone S18018E) PE/Cyanine7 (left) or rat IgG2b, κ PE/Cyanine7 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
162311 | 25 µg | 65 CHF | ||||
162312 | 100 µg | 195 CHF |
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD8a
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_3083284 (BioLegend Cat. No. 162311)
AB_3083284 (BioLegend Cat. No. 162312)
Antigen Details
- Structure
- Ig superfamily, CD8α chain, 34 kD
- Distribution
-
Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells
- Function
- Co-receptor for TCR
- Ligand/Receptor
- MHC class I molecule
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
8. Kamimura D, et al. 2006. J. Immunol. 177:306.
9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590 - Gene ID
- 12525 View all products for this Gene ID
- UniProt
- View information about CD8alpha on UniProt.org
Related FAQs
Other Formats
View All CD8a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD8a Antibody | S18018E | FC |
PE anti-mouse CD8a | S18018E | FC |
APC anti-mouse CD8a | S18018E | FC |
APC/Fire™ 750 anti-mouse CD8a | S18018E | FC |
PerCP/Cyanine5.5 anti-mouse CD8a | S18018E | FC |
PE/Cyanine7 anti-mouse CD8a | S18018E | FC |
FITC anti-mouse CD8a | S18018E | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD8a Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD3e ... -
PE anti-mouse CD8a
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ɛ ... -
APC anti-mouse CD8a
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
APC/Fire™ 750 anti-mouse CD8a
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
PerCP/Cyanine5.5 anti-mouse CD8a
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
PE/Cyanine7 anti-mouse CD8a
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
FITC anti-mouse CD8a
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ...
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