- Clone
- W16125D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- interleukin27 receptor alpha, IL27ra, IL-27Ra, TCCR, WSX-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W16125D_PE_CYANINE7_IL-27Ralpha_Antibody_021822 W16125D_PE_CYANINE7_IL-27Ralpha_Antibody_021822](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W16125D_PE_CYANINE7_IL-27Ralpha_Antibody_021822.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were surface stained with anti-mouse CD3 FITC and anti-mouse IL-27Rα (clone W16125D) PE/Cyanine7 (left) or rat IgG2a, κ PE/Cyanine7 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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159005 | 25 µg | 188 CHF | ||||
159006 | 100 µg | 457 CHF |
69 kD glycosylated transmembrane subunit of IL-27 receptor complex consisting of IL27Ra (WSX-1, TCCR) and gp130 (CD130). Expressed on T cells, monocytes, and neutrophils and aside from its effect on innate immunity, is involved in dampening the extent of Th1 immune response post infection. Activity levels of INF-γ and IL-17 producing T cells are regulated through this receptor which relays signal through Jak-STAT pathway.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse IL-27Rα transfectant
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
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AB_2910326 (BioLegend Cat. No. 159005)
Antigen Details
- Distribution
-
IL27Ra is expressed by T cells, monocytes, and neutrophils.
- Ligand/Receptor
- IL27Ra combines with gp130 to form a receptor complex whose activation by IL-27 leads to differing pathway-dependent effector activities.
- Cell Type
- Monocytes, Neutrophils, T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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- Takeda A, et al. 2003. J Immunol. 170:4886-90.
- Yoshida H & Hunter CA. 2015. Annu Rev Immunol. 33:417-43.
- Iwasaki Y, et al. 2015. Int J Mol Sci. 16:2851-63.
- Gene ID
- 50931 View all products for this Gene ID
- UniProt
- View information about IL-27Ralpha on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All IL-27Rα Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse IL-27Rα | W16125D | FC |
PE anti-mouse IL-27Rα | W16125D | FC |
PE/Cyanine7 anti-mouse IL-27Rα | W16125D | FC |
PE/Dazzle™ 594 anti-mouse IL-27Rα | W16125D | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse IL-27Rα
C57BL/6 splenocytes were stained with CD3 APC and purified I... C57BL/6 splenocytes were stained with CD130 purified (follow... -
PE anti-mouse IL-27Rα
C57BL/6 splenocytes were stained with anti-mouse CD3ε Brilli... -
PE/Cyanine7 anti-mouse IL-27Rα
C57BL/6 mouse splenocytes were surface stained with anti-mou... -
PE/Dazzle™ 594 anti-mouse IL-27Rα
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ɛ ...
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