- Clone
- SA376A4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- My10, gp105-120, mucosialin
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![SA376A4_PEDazzle594_Antibody_110121 SA376A4_PEDazzle594_Antibody_110121](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/SA376A4_PEDazzle594_Antibody_110121.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were stained with anti-mouse Lineage Cocktail Alexa Fluor® 700 and CD34 (clone SA376A4) PE/Dazzle™ 594 (left) or Rat IgG2a, κ PE/Dazzle™ 594 isotype control (right). Data shown was gated on the total cell population.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
152209 | 25 µg | 171 CHF | ||||
152210 | 100 µg | 375 CHF |
CD34, also known as gp105-120, is a highly glycosylated type I monomeric sialomucin-like glycophophoprotein with an approximate molecular weight of 105-120 kD, with two generated by alternative splicing. CD34 is expressed on hematopoietic progenitors, endothelial cells, brain, and testis. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands, and mediates the attachment of stem cells to the extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse CD34 transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm. - Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This antibody (clone SA376A4) is useful for staining mouse CD34+ hematopoietic stem cell but it does not stain NIH-3T3 cells and the staining of Bend.3 cells is dimmer compared to other mouse CD34 antibodies, probably due to this antibody recognizes a different epitope than the other monoclonal antibodies.
- RRID
-
AB_2734218 (BioLegend Cat. No. 152209)
AB_2734219 (BioLegend Cat. No. 152210)
Antigen Details
- Structure
- Type I monomeric glycophophoprotein with an approximate molecular weight of 105-120 kD.
- Distribution
-
Hematopoietic progenitors, endothelial cells, brain and testis tissues.
- Function
- Cell adhesion and homing.
- Interaction
- PKC.
- Ligand/Receptor
- L-selectin and E-selectin.
- Cell Type
- Endothelial cells, Hematopoietic stem and progenitors
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Garlanda C, et al. 1997. Eur J Cell Biol. 73:368-77.
2. Brown J, et al. 1991. Int Immunol. 3:175.
3. Suda J, et al. 1992. Blood. 79:2288-95.
4. Baumhueter S, et al. 1994. Blood. 84:2554. - Gene ID
- 12490 View all products for this Gene ID
- UniProt
- View information about CD34 on UniProt.org
Related FAQs
Other Formats
View All CD34 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse CD34 | SA376A4 | FC |
Alexa Fluor® 647 anti-mouse CD34 | SA376A4 | FC |
Brilliant Violet 421™ anti-mouse CD34 | SA376A4 | FC |
PE/Dazzle™ 594 anti-mouse CD34 | SA376A4 | FC |
TotalSeq™-C0823 anti-mouse CD34 | SA376A4 | PG |
TotalSeq™-B0823 anti-mouse CD34 Antibody | SA376A4 | PG |
APC anti-mouse CD34 | SA376A4 | FC |
PE/Cyanine7 anti-mouse CD34 | SA376A4 | FC |
TotalSeq™-A0823 anti-mouse CD34 | SA376A4 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD34
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Alexa Fluor® 647 anti-mouse CD34
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Brilliant Violet 421™ anti-mouse CD34
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Dazzle™ 594 anti-mouse CD34
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
TotalSeq™-C0823 anti-mouse CD34
-
TotalSeq™-B0823 anti-mouse CD34 Antibody
-
APC anti-mouse CD34
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
PE/Cyanine7 anti-mouse CD34
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
TotalSeq™-A0823 anti-mouse CD34
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