- Clone
- TS2/4 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V S160
- Other Names
- LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![TS2-4_PerCPCy55_CD11a_Antibody_041018 TS2-4_PerCPCy55_CD11a_Antibody_041018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/TS2-4_PerCPCy55_CD11a_Antibody_041018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with PerCP/Cyanine5.5 CD11a (clone TS2/4; filled histogram) or PerCP/Cyanine5.5 mouse IgG1, κ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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350613 | 25 tests | 176 CHF | ||||
350614 | 100 tests | 382 CHF |
CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HLA-DR CTL line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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Additional reported applications (for relevant formats) include: immunoprecipitation1, 2. Note: the antibody TS2/4 recognizes the ß-propeller domain of CD11a and only immunoprecipitates the CD11a/CD18 complex.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Huang C and Springer TA. 1997. P. Natl. Acad. Sci. USA 94:3162. (IP)
- Sanchez-Madrid F, et al. 1983. J. Exp. Med. 158:1785. (IP)
- Sanchez-Madrid F, et al. 1982. P. Natl. Acad. Sci. USA 79:7489. (FC)
- RRID
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AB_2728320 (BioLegend Cat. No. 350613)
AB_2728321 (BioLegend Cat. No. 350614)
Antigen Details
- Structure
- Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
- Distribution
-
Leukocytes
- Function
- Adhesion, costimulation
- Ligand/Receptor
- ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146. - Gene ID
- 3683 View all products for this Gene ID
- UniProt
- View information about CD11a on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD11a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD11a | TS2/4 | FC,IP |
FITC anti-human CD11a | TS2/4 | FC |
PE anti-human CD11a | TS2/4 | FC |
PerCP anti-human CD11a | TS2/4 | FC |
PE/Cyanine7 anti-human CD11a | TS2/4 | FC |
APC/Fire™ 750 anti-human CD11a | TS2/4 | FC |
PerCP/Cyanine5.5 anti-human CD11a | TS2/4 | FC |
TotalSeq™-A0185 anti-human CD11a | TS2/4 | PG |
TotalSeq™-C0185 anti-human CD11a | TS2/4 | PG |
TotalSeq™-B0185 anti-human CD11a | TS2/4 | PG |
TotalSeq™-D0185 anti-human CD11a | TS2/4 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD11a
Human peripheral blood lymphocytes stained with PE anti-huma... -
FITC anti-human CD11a
Human peripheral blood lymphocytes stained with FITC anti-hu... -
PE anti-human CD11a
Human peripheral blood lymphocytes stained with PE anti-huma... Pre-lysed human blood leukocytes were stained with PE anti-h... -
PerCP anti-human CD11a
Human peripheral blood lymphocytes stained with PerCP anti-h... -
PE/Cyanine7 anti-human CD11a
Human peripheral blood lymphocytes were stained with PE/Cyan... -
APC/Fire™ 750 anti-human CD11a
Human peripheral blood lymphocytes were stained with APC/Fir... -
PerCP/Cyanine5.5 anti-human CD11a
Human peripheral blood lymphocytes were stained with PerCP/C... -
TotalSeq™-A0185 anti-human CD11a
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TotalSeq™-C0185 anti-human CD11a
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TotalSeq™-B0185 anti-human CD11a
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TotalSeq™-D0185 anti-human CD11a
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