- Clone
- QA21A24 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD45, Leukocyte Common Antigen (LCA), T200
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA21A24_PerCPCyanine5pt5_CD45_Recom_Antibody_100923 QA21A24_PerCPCyanine5pt5_CD45_Recom_Antibody_100923](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA21A24_PerCPCyanine5pt5_CD45_Recom_Antibody_100923.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD45 recombinant (clone QA21A24) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, к PerCP/Cyanine5.5 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
384409 | 25 tests | 135 CHF | ||||
384410 | 100 tests | 330 CHF |
CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
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Blue Laser (488 nm)
- RRID
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AB_3083385 (BioLegend Cat. No. 384409)
AB_3083385 (BioLegend Cat. No. 384410)
Antigen Details
- Structure
- Type I transmembrane protein, 180 – 240 kD (multiple isoforms)
- Distribution
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Hematopoietic cells, except circulating erythrocytes or platelets
- Function
- Tyrosine phosphatases, signaling
- Cell Type
- B cells, Granulocytes, Leukocytes, Lymphocytes, Monocytes, T cells
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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- Thomas M. 1989. Annu Rev Immunol. 7:339-69.
- Trowbridge I, et al. 1994. Annu Rev Immunol. 12:85-116.
- Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All CD45 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-human CD45 Recombinant Antibody | QA21A24 | FC |
APC anti-human CD45 Recombinant Antibody | QA21A24 | FC |
FITC anti-human CD45 Recombinant Antibody | QA21A24 | FC |
PE anti-human CD45 Recombinant Antibody | QA21A24 | FC |
PerCP/Cyanine5.5 anti-human CD45 Recombinant Antibody | QA21A24 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD45 Recombinant Antibody
Human peripheral blood lymphocytes were stained with purifie... -
APC anti-human CD45 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD45 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human CD45 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD45 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...
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