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PerCP/Cyanine5.5 anti-mouse CD36 Antibody

Pricing & Availability
Clone
HM36 (See other available formats)
Regulatory Status
RUO
Other Names
FAT, gpIIIb, gpIV
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
HM36_PerCPCyanine55_CD36_1_Antibody_082218
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with CD36 (clone HM36) PerCP/Cyanine5.5 and CD11b (clone M1/70) APC (left) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control and CD11b (clone M1/70) APC (right).
  • HM36_PerCPCyanine55_CD36_1_Antibody_082218
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were stained with CD36 (clone HM36) PerCP/Cyanine5.5 and CD11b (clone M1/70) APC (left) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control and CD11b (clone M1/70) APC (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
102619 25 µg 165 CHF
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102620 100 µg 370 CHF
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Description

CD36 is a 85 kD glycoprotein, also known as FAT, gpIIIb, or gpIV. It is a member of the class B scavenger receptor family, expressed on platelets, monocytes, macrophages, megakaryocytes, microvasculature, dendritic cells and mammary endothelial cells. The primary ligands for CD36 have been reported to be oxidized low density lipoprotein, anionic phospholipids, and collagens I, IV, and V. CD36 acts as a scavenger receptor thus promoting the removal of apoptotic neutrophils and other apoptotic bodies, as well as clearance of defective erythrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Full length version of the protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Hamilton R F, 2006. J. Biol. Chem. 281:34218.
  2. Stuart L M, 2007. J. Biol. Chem. doi:10.1074/jbc.M702887200.
  3. Hu Q, 2018. Nat Biomed Eng. 2(11): 831.
Product Citations
  1. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  2. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
RRID
AB_2750187 (BioLegend Cat. No. 102619)
AB_2750188 (BioLegend Cat. No. 102620)

Antigen Details

Structure
Scavenger receptor superfamily, 85 kD
Distribution

Platelets, monocytes, macrophages, microvascular and mammary endothelial cells

Function
Scavenger receptor, recognition and phagocytosis of apoptotic neutrophils, adhesion
Ligand/Receptor
Oxidized low density lipoprotein, anionic phospholipids, collagen I, IV, and V
Cell Type
Endothelial cells, Macrophages, Monocytes, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Greenwalt DE, et al. 1992. Blood 80:1105.
3. Endemann G, et al. 1993. J. Biol. Chem. 268:11811.

Gene ID
12491 View all products for this Gene ID
UniProt
View information about CD36 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 08.22.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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