- Clone
- A17067B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Apolipoprotein E, Alzheimer Disease 2, LDLCQ5, LPG, AD2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A17067B_Pure_APOE_Antibody_1_040518 A17067B_Pure_APOE_Antibody_1_040518](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A17067B_Pure_APOE_Antibody_1_040518.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-APO E, 109-116 antibody (clone A17067B). Lane 1: Molecular weight marker; Lane2: 50 ng of recombinant human Apo E2; Lane 3: 50 ng of recombinant human Apo E3; Lane 4: 50 ng of recombinant human Apo E4; Lane 5: 16 µg of human plasma. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system. -
Direct ELISA of purified anti-APO E, 109-116 antibody (clone A17067B) binding to plate-immobilized recombinant human APO E2, APO E3, and APO E4. ELISA was performed by coating wells with 100 ng of APO E proteins. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
852701 | 25 µg | 112 CHF | ||||
852702 | 100 µg | 276 CHF |
Apolipoprotein E (Apo E) belongs to a class of apolipoproteins that mediate the binding, internalization, and catabolism of lipoprotein particles. Apo E is produced by the liver and macrophages in peripheral tissues where it regulates cholesterol metabolism. In the central nervous system, Apo E is primarily produced by astrocytes and plays an important role in transporting cholesterol to neurons via Apo E receptors. Apo E has three alleles (ApoE2, ApoE3 and ApoE4) that differ from each other by only one or two amino acids at positions 112 and 158. ApoE 4 is one of the largest known genetic risk factors for late-onset Alzheimer's disease. Furthermore, Apo E is associated with cardiovascular disease, Lipoprotein Glomerulopathy and Sea-Blue Histiocyte Disease.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Direct ELISA - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For Direct ELISA applications, a concentration range of 0.004 - 0.4 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
- Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47.
- Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23.
- Kim J, et al. 2014. Mol. Cells. 37:767
- Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
- RRID
-
AB_2728597 (BioLegend Cat. No. 852701)
AB_2728598 (BioLegend Cat. No. 852702)
Antigen Details
- Structure
- Human APO E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major isoforms, APO E2, APO E3, and APO E4.
- Distribution
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Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney.
Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.
- Function
- Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.
- Interaction
- Tau, amyloid beta
- Ligand/Receptor
- LDL receptor, SORL1
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Apolipoproteins
- Antigen References
-
- Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
- Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47.
- Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23.
- Kim J, et al. 2014. Mol. Cells. 37:767
- Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312
- Gene ID
- 348 View all products for this Gene ID
- UniProt
- View information about Apo E 109-116 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Apo E, 109-116 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Apo E, 109-116 | A17067B | WB,Direct ELISA |
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