- Clone
- W16197A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ACTB, Actin, cytoplasmic 1, Beta-actin
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W16197A_PURE_beta-actin_Anitbody_1_101217 W16197A_PURE_beta-actin_Anitbody_1_101217](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W16197A_PURE_beta-actin_Anitbody_1_101217.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain and zebra fish were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 diluted (0.1 µg/mL) purified anti-β-actin antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Lane M: MW ladder. -
Total lysates (15 µg protein) from HeLa was resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:800 diluted (0.25 µg/mL), 1:1600 diluted (0.125 µg/mL), 1:3200 diluted (0.0625 µg/mL), 1:6400 diluted (0.03125 µg/mL) and 1:12800 diluted (0.0155 µg/mL) purified anti-β-actin antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Lane M: MW ladder. -
Western blot of purified anti-β-actin antibody (clone W16197A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
664801 | 25 µg | 101 CHF | ||||
664802 | 100 µg | 253 CHF |
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, Zebrafish, Drosophila
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human ACTB peptide (1-13 a.a.).
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.1 µg/ml in human, mouse, rat and zebrafish and 0.1 - 0.5 µg/ml in Drosophila. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Reactivity to Drosophila was only verified with the purified format.
- Product Citations
-
- RRID
-
AB_2721350 (BioLegend Cat. No. 664801)
AB_2721349 (BioLegend Cat. No. 664802)
Antigen Details
- Structure
- 375 amino acids with a predicted molecular weight of 42 kD.
- Distribution
-
Cytoplasm
- Function
- Actins are highly conserved proteins that are involved in cell motility.
- Interaction
- Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Antigen References
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1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687. - Gene ID
- 60 View all products for this Gene ID
- UniProt
- View information about beta-actin on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All β-actin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-β-actin | W16197A | WB |
Direct-Blot™ HRP anti-β-actin | W16197A | WB |
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Purified anti-β-actin
Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain a... Total lysates (15 µg protein) from HeLa was resolved by elec... Western blot of purified anti-β-actin antibody (clone W16197... -
Direct-Blot™ HRP anti-β-actin
Whole cell lysates (15 µg protein) from HeLa was resolved by...
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