- Clone
- 6H2.B4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cyt c
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_6H2.B4_PURE_CytochromeC_Antibody_1_IP_090916 1_6H2.B4_PURE_CytochromeC_Antibody_1_IP_090916](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6H2.B4_PURE_CytochromeC_Antibody_1_IP_090916.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Immunoprecipitation/Western Blot analysis using purified anti-Cytochrome c antibody (6H2.B4). 800 µg of HeLa cell lysates was tested at protein concentration of 1µg/µl for each sample. Lane 1 was immunoprecipitated with control antibody and lane 2 was immunoprecipitated with anti-Cytochrome c Antibody (6H2.B4). Western blot analysis was performed using anti-Cytochrome c Antibody (7H8.2C12). -
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified cytochrome c (clone 6H2.B4) in blocking buffer overnight at 4°C, and followed by DyLight™ 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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612301 | 25 µg | 83 CHF | ||||
612302 | 100 µg | 202 CHF |
Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat cyt c-OVA
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
- Application
-
IP - Quality tested
ICC - Verified
ICFC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 2.0 - 4.0 µg /1 x107 cell equivalents for immunoprecipitation. For Western blot application, the suggested use of this reagent is 0.5 µg per ml (1:1000 dilution). For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
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Application References
(PubMed link indicates BioLegend citation) -
- Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134.
- Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143.
- Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF)
- Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP)
- Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF)
- She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed
- McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed
- Product Citations
-
- RRID
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AB_315774 (BioLegend Cat. No. 612301)
AB_315775 (BioLegend Cat. No. 612302)
Antigen Details
- Structure
- Heme binding domain; 15 kD
- Distribution
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Mitochondrial intermembrane space
- Function
- Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
- Interaction
- Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Mitochondrial Markers
- Antigen References
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1. Liu X, et al. 1996. Cell. 86:147.
2. Li P, et al. 1997. Cell. 91:479.
3. Zhang Z, et al. 2003. Gene 312:61.
4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793. - Gene ID
- 1355 View all products for this Gene ID
- UniProt
- View information about Cytochrome c on UniProt.org
Other Formats
View All Cytochrome c Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-Cytochrome c | 6H2.B4 | ICFC |
FITC anti-Cytochrome c | 6H2.B4 | ICFC |
Purified anti-Cytochrome c | 6H2.B4 | IP,ICC,ICFC |
Alexa Fluor® 488 anti-Cytochrome c | 6H2.B4 | ICFC |
Alexa Fluor® 647 anti-Cytochrome c | 6H2.B4 | ICFC,ICC |
FITC anti-Cytochrome c | 6H2.B4 | FC |
GMP FITC anti-Cytochrome c | 6H2.B4 | FC |
TotalSeq™-B0043 anti-Cytochrome c | 6H2.B4 | ICPG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-Cytochrome c
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FITC anti-Cytochrome c
Balb/c splenocytes intracellularly stained with 6H2.B4 FITC -
Purified anti-Cytochrome c
Immunoprecipitation/Western Blot analysis using purified ant... HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10... -
Alexa Fluor® 488 anti-Cytochrome c
C57BL/6 splenocytes were treated with BioLegend's Fixation B... -
Alexa Fluor® 647 anti-Cytochrome c
C57BL/6 splenocytes were treated with BioLegend's Fixation B... A-431 cells were fixed with 4% paraformaldehyde for 10 minut... -
FITC anti-Cytochrome c
Typical quality control results from intracellular staining ... -
GMP FITC anti-Cytochrome c
Typical quality control results from intracellular staining ... -
TotalSeq™-B0043 anti-Cytochrome c
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