- Clone
- NT63 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
- Isotype
- Mouse IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![NT63_PURE_RNA_PolymeraseBeta_WB_102717_updated NT63_PURE_RNA_PolymeraseBeta_WB_102717_updated](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/NT63_PURE_RNA_PolymeraseBeta_WB_102717_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase β antibody (clone NT63). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: Molecular weight ladder. -
Total cell lysate from E Coli. Strain BL21 (15 µg protein) was resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL (lane 1), 0.05 µg/mL (lane 2), 0.025 µg/mL (lane 3), 0.0125 µg/mL (lane 4) and 0.00625 µg/mL (lane 5) of Purified anti-E. coli RNA Polymerase β Antibody (clone NT63) (upper blot). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated anti-mouse-IgG secondary antibody for anti-E. coli RNA Polymerase β Antibody (upper blot). Ponceau S staining (lower blot) was used as loading control. Lane M: Molecular Weight Marker
Cat # | Size | Price | Quantity Check Availability | Save | ||
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663006 | 100 µg | 323 CHF |
RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the rpoB gene. RNA polymerase β is a subunit which, along with α (rpoC), limits core polymerase expression.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified protein.
- Formulation
- Phosphate-buffered solution, pH 7.2.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
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WB - Quality tested
ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.00625 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone NT63 recognizes epitope 922-1090.
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Application References
(PubMed link indicates BioLegend citation) -
- Thompson NE, et al. 1992. Biochemistry 31:7003.
- Product Citations
-
- RRID
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AB_2565555 (BioLegend Cat. No. 663006)
Antigen Details
- Structure
- 1252 amino acids with a predicted molecular weight of approximately 150 kD.
- Distribution
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Bacteria.
- Function
- Transcriptional regulator in bacteria.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
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1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerard Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature 451:851. - Gene ID
- 948488 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase beta on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RNA Polymerase β Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-E. coli RNA Polymerase β | NT63 | WB,ELISA |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-E. coli RNA Polymerase β
Total lysates (15 µg protein) from E. coli BL21 cells were r... Total cell lysate from E Coli. Strain BL21 (15 µg protein) w...
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