- Clone
- W19080B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein Disulfide Isomerase Family A Member 3, PDIA3, Endoplasmic Reticulum Resident Protein 57, GRP58, ER Protein 57, GRP57, Epididymis Secretory Protein Li 269, Epididymis Scretory Sperm Binding Protein Li 93n
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W19080B_PURE_ERp57_Antibody_1_061020 W19080B_PURE_ERp57_Antibody_1_061020](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W19080B_PURE_ERp57_Antibody_1_061020.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-ERp57 antibody (clone W19080B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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937301 | 25 µg | 101 CHF | ||||
937302 | 100 µg | 253 CHF |
ERp57 is a member of the protein disulfide family of endoplasmic reticulum localized oxidoreductases and plays an important role in disulfide bond formation and protein folding. Along with calnexin and calreticulin, it is essential for the synthesis and quality control of nascent glycoproteins in the secretory pathway. It is also a core component of the peptide-loading complex that is required for MHC class I presentation. ERp57 is upregulated in multiple neurodegenerative diseases and cancers, and targeted disruption of ERp57 sensitizes cancer cells to chemotherapeutic agents and ionizing radiation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human ERp57 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone failed IP testing.
- Product Citations
-
- RRID
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AB_2861126 (BioLegend Cat. No. 937301)
AB_2861127 (BioLegend Cat. No. 937302)
Antigen Details
- Structure
- ERp57 is a 505 amino acid protein with a predicted molecular weight of 57 kD.
- Distribution
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Endoplasmic reticulum/Ubiquitously expressed
- Function
- Molecular chaperone
- Biology Area
- Cell Biology, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
- Molecular Family
- Endoplasmic Reticulum Markers, Enzymes and Regulators
- Antigen References
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- Oliver JD, et al. 1997. Science. 275:86-8.
- Kienast A, et al. 2007. Nat Immunol. 8:864-72.
- Hussman M, et al. 2015. Oncotarget. 6:39247-61.
- Blees A, et al. 2017. Nature. 551:525-528.
- Gene ID
- 2923 View all products for this Gene ID
- UniProt
- View information about ERp57 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All ERp57 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-ERp57 | W19080B | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-ERp57
Whole cell extracts (15 µg total protein) from the indicated...
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