- Clone
- W15183A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Glycogen synthase kinase alpha, GSK-3 alpha
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W15183A_PURE_GSK-3alpha_Antibody_1_032019 W15183A_PURE_GSK-3alpha_Antibody_1_032019](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W15183A_PURE_GSK-3alpha_Antibody_1_032019.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg total protein) from HEK293T (WT) and HEK293T GSK3α KD cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-GSK3α Antibody, clone W15183A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. -
Total cell lysates (15 µg total protein) from HeLa (reduced expression control), HEL, U87-MG and A549 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-GSK3α Antibody, clone W15183A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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688902 | 100 µg | 288 CHF |
Glycogen Synthase Kinase 3 Alpha (GSK-3α) is a serine/threonine protein kinase which mediates the control of several regulatory proteins involved in glucose homeostasis, including glycogen synthase, and transcription factors such as JUN. It also plays a role in the WNT and PI3K signaling pathways. GSK-3α contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting glycogen synthase (GYS1 or GYS2) activity and mediates the development of insulin resistance. In Wnt signaling, GSK-3α regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. GSK-3α is also reported to facilitate the generation of amyloid precursor protein (APP)-derived amyloid plaques found in Alzheimer disease. To date, there are six GSK-3α mutant mouse lines generated, these stains express various abnormal phenotypes in growth, size, mortality, aging, adipose morphology, homeostasis and metabolism, muscle and the nervous system.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial human GSK-3α recombinant protein (251-483 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
KO/KD-WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2629652 (BioLegend Cat. No. 688902)
Antigen Details
- Structure
- 483 amino acids with a predicted molecular weight of 51 kD. GSK-3α is a serine/threoenine kinase.
- Distribution
-
Cytoplasm.
- Function
- GSK-3α is a serine/threonine kinase, mediating signals in the WNT and the PI3K pathways. GSK-3α is implicated in the regulation of glucose metabolism and homeostasis.
- Interaction
- Interacts with ARRB2, AXIN1, CTNNB1, and CTNND2.
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Park SA, et al. 2016. PLoS One 11:e0153075.
2. Ye C, et al. 2015. J. Neurochem. 133:273.
3. Grassilli E, et al. 2014. PLoS One 9:e100947.
4. Nekova TS, et al. 2014. Br. J. Haematol. 167:272.
5. Zhou J, et al. 2013. J. Clin. Invest. 123:1821.
6. Jaworski T, et al. 2011. Nature 480:e4. - Gene ID
- 2931 View all products for this Gene ID
- UniProt
- View information about GSK-3alpha on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All GSK-3α Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GSK-3α | W15183A | WB,KO/KD-WB |
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