- Clone
- A19237B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Autophagy Related 3
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A19237B_PURE_ATG3_Antibody_052322 A19237B_PURE_ATG3_Antibody_052322](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A19237B_PURE_ATG3_Antibody_052322-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ATG3 (clone A19237B) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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379702 | 100 µg | 288 CHF |
Human autophagy related 3, ATG3, is a 314 amino acid protein with a molecular mass of 36 kD, which is a ubiquitin-like modifier similar to the canonical E2 enzyme. ATG3 is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy is the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway which plays an important role in controlling normal growth but may be defective in tumor cells. ATG3 is essential for autophagocytosis, which is a starvation-induced process responsible for transport of cytoplasmic proteins to the lysosome/vacuole.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2922609 (BioLegend Cat. No. 379702)
Antigen Details
- Structure
- Human ATG3 is a 314 amino acid protein with a molecular mass of 36 kD
- Distribution
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Expressed in testis and bone marrow stromal cell
- Function
- E2-conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2, or MAP1LC3A).
- Interaction
- Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
- Ligand/Receptor
- ATG8
- Biology Area
- Cell Biology, Cell Death, Immunology
- Molecular Family
- Apolipoproteins, Autophagosome Markers
- Antigen References
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- Isei T, et al. 2002. J Biol Chem. 277:13739.
- Silke M. et al. 2009. Hum Genet. 128:453.
- Ozlem O, et al. 2012. Apoptosis. 17:810.
- Gene ID
- 64422 View all products for this Gene ID
- UniProt
- View information about ATG3 on UniProt.org
Related FAQs
Other Formats
View All ATG3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human ATG3 | A19237B | IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human ATG3
Human paraffin-embedded testis tissue slices were prepared w...
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