- Clone
- L1-OV198.5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CAML1, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, S10, SPG1, L1CAM, Neural cell adhesion molecule L1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_L1dashOV198point5_PURE_CD171_Antibody_1_FC_041216 1_L1dashOV198point5_PURE_CD171_Antibody_1_FC_041216](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L1dashOV198point5_PURE_CD171_Antibody_1_FC_041216.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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M21 cells were stained with purified CD171 (clone L1-OV198.5, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE. -
HeLa cells were fixed with 2% PFA for 10 minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were stained with 2 µg/mL anti-CD171 Antibody (clone L1-OV198.5) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective. -
Total cell lysates (15 µg protein) from HeLa, HepG2 and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-CD171 Antibody (clone L1-OV198.5). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection. HDAC1 Antibody was used as loading control.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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371602 | 100 µg | 276 CHF |
CD171, also known as L1CAM or L1, is a transmembrane glycoprotein. It is a 200-220 kD neuronal cell adhesion molecule and a member of the L1 protein family belonging to the immunoglobulin superfamily.
This cell adhesion molecule plays an important role in nervous system development, including neuron-neuron adhesion, signal transduction, axon guidance, cell migration and differentiation. CD171 also has a strong implication in treatment-resistanct cancers. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- SKOV-3 ovarian carcinoma cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, WB - Verified
IHC-F, IHC-P - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
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AB_2616786 (BioLegend Cat. No. 371602)
Antigen Details
- Structure
- 200-220 kD. Several immunoglobulin-like domains and fibronectin-like repeats (type III), linked via a single transmembrane sequence to a conserved cytoplasmic domain.
- Distribution
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Tetanus-toxin positive neurons, endothelial cells, certain epithelial cells, reticular fibroblasts, colon and breast carcinomas, colon melanoma, tumor cells of neuronal and mesothelial origin.
- Function
- Adhesion molecule. Homotypic and heterotypic cell-cell interaction in neuronal myelination, neurite outgrowth and regeneration. Axonal guidance, neuronal migration.
- Ligand/Receptor
- Homophilic interaction with itself and heterophilic binding to integrins, other cell adhesion molecules, extracellular matrix molecules.
- Cell Type
- Endothelial cells, Epithelial cells, Fibroblasts, Neurons
- Biology Area
- Cancer Biomarkers, Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Wolterink S, et al. 2010. Cancer Res. 70(6):2504.
2. Huszar M, et al. 2006. Human Pathology 37(8):1000.
3. Kaifi JT, et al. 2006. Anticancer Res. 26(2A):1167.
4. Kaifi JT, et al. 2006. Mod. Pathol. 3:399.
5. Meier F, et al. 2006. Int. J. Cancer 119(3)549.
6. Stoeck A, et al. 2006. Biochem. J. 393:609.
7. Gutwein P, et al. 2005. Clin. Cancer Res. 11(7)2492. - Gene ID
- 3897 View all products for this Gene ID
- UniProt
- View information about CD171 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD171 (L1CAM) Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-human CD171 (L1CAM) | L1-OV198.5 | FC,ICC,WB,IHC-F,IHC-P |
PE anti-human CD171 (L1CAM) | L1-OV198.5 | FC |
Alexa Fluor® 647 anti-human CD171 (L1CAM) | L1-OV198.5 | FC,IHC-P |
TotalSeq™-C0430 anti-human CD171 (L1CAM) | L1-OV198.5 | PG |
TotalSeq™-B0430 anti-human CD171 (L1CAM) | L1-OV198.5 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD171 (L1CAM)
M21 cells were stained with purified CD171 (clone L1-OV198.5... HeLa cells were fixed with 2% PFA for 10 minutes, permeabili... Total cell lysates (15 µg protein) from HeLa, HepG2 and NIH3... -
PE anti-human CD171 (L1CAM)
M21 cells were stained with CD171 (clone L1-OV198.5) PE (fil... -
Alexa Fluor® 647 anti-human CD171 (L1CAM)
Human M21 cell line was stained with CD171 (clone L1-OV198.5... Human paraffin-embedded colon tissue slices were prepared wi... -
TotalSeq™-C0430 anti-human CD171 (L1CAM)
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TotalSeq™-B0430 anti-human CD171 (L1CAM)
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