- Clone
- IRF4.3E4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon regulatory factor 4
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_IRF43E4_Purified_IRF4_Purified_Antibody_WB_050918 1_IRF43E4_Purified_IRF4_Purified_Antibody_WB_050918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/IRF43E4_Purified_IRF4_Antibody_WB_050918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Total lysates (15 µg protein) from HeLa (lane 1, negative control) and Ramos (lane 2) were resolved by electrophoresis (4-12% Bis-Tris midi gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) Purified anti-IRF4 Antibody, clone IRF4.3E4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-rat-IgG secondary antibody conjugated to HRP for the anti-IRF4 Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder. -
Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from U266 cells treated with IFNα2 with either A) 1:200 dilution of Go-ChIP-Grade™ Purified anti-IRF4 (Clone IRF4.3E4), or B) equal amount of Purified Rat IgG1, κ Isotype Control Antibody (Cat. No. 400401). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human SUB1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
646402 | 100 µg | 265 CHF |
The IRF family consists of at least nine members. IRF4 and IRF8 are highly homologous to each other and also redundant in function. IRF4 is critical for Th2 and Th17 development. Together with TRF8, it plays an essential role in macrophage and dendritic cell development and function. IRF4 is also reported to be essential for pre-B cell development, receptor editing, germinal center reactor and plasma cell differentiation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- GST fusion protein containing C-terminal of murine IRF4
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ChIP - Verified
ICFC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg/ml. For ChIP applications, the suggested dilution is is 1:50-1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2280462 (BioLegend Cat. No. 646402)
Antigen Details
- Structure
- Molecular weight is approximately 51 kD.
- Distribution
-
IRF4 is reported to be expressed exclusively in the immune system.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Lu R. 2008. Trends Immunol 29:487.
- Gene ID
- 3662 View all products for this Gene ID
- UniProt
- View information about IRF4 on UniProt.org
Other Formats
View All IRF4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 488 anti-IRF4 | IRF4.3E4 | ICFC |
Purified anti-IRF4 | IRF4.3E4 | WB,ICFC,ChIP |
PE anti-IRF4 | IRF4.3E4 | ICFC,SB |
Alexa Fluor® 647 anti-IRF4 | IRF4.3E4 | ICFC |
PE/Cyanine7 anti-IRF4 | IRF4.3E4 | ICFC |
PerCP/Cyanine5.5 anti-IRF4 | IRF4.3E4 | ICFC |
Alexa Fluor® 594 anti-IRF4 | IRF4.3E4 | ICC |
Pacific Blue™ anti-IRF4 | IRF4.3E4 | ICFC |
Customers Also Purchased
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/11-26Cdot2a_Pure_091907.jpg)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/m5e2.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Alexa Fluor® 488 anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... -
Purified anti-IRF4
Total lysates (15 µg protein) from HeLa (lane 1, negative co... Chromatin Immunoprecipitation (ChIP) was performed using Go-... -
PE anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... Confocal image of human lymph node sample acquired using the... -
Alexa Fluor® 647 anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... -
PE/Cyanine7 anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... -
PerCP/Cyanine5.5 anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral... -
Alexa Fluor® 594 anti-IRF4
A20 cells were fixed with 4% paraformaldehyde (PFA) for 10 m... -
Pacific Blue™ anti-IRF4
PHA-stimulated (3 days) or freshly isolated human peripheral...
Follow Us