- Clone
- N1/12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SLC12A5, rKCC2, K-Cl cotransporter 2, neuronal K-Cl cotransporter, solute carrier family 12, member 5, neuronal-specific K-Cl cotransporter, furosemide-sensitive K-Cl cotransporter, electroneutral potassium-chloride cotransporter 2
- Previously
-
Covance Catalog# MMS-5241
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N1slash12_Purified_KCC2_Antibody_1_021918 N1slash12_Purified_KCC2_Antibody_1_021918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N1slash12_Purified_KCC2_Antibody_1_021918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-KCC2 antibody (clone LN1/12). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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821701 | 100 µL | 165 CHF |
Potassium-chloride cotransporter member 5 (also known as KCC2 and SCL12A5) is responsible for establishing the chloride ion gradient in neurons through the maintenance of low intracellular chloride concentrations. Mediates synaptic inhibition, protects against excitotoxicity and modulates plasticity.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2564853 (BioLegend Cat. No. 821701)
Antigen Details
- Structure
- Expected MW: 140 kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 171373 View all products for this Gene ID
- UniProt
- View information about KCC2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All KCC2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-KCC2 | N1/12 | WB,IHC |
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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