- Clone
- STOP (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Microtubule-associated protein 6, MAP-6, stable tubule-only polypeptide
- Previously
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Covance Catalog# MMS-5104
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_STOP_Purified_MAP6_Anitbody_3_123118.png 1_STOP_Purified_MAP6_Anitbody_3_123118.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/STOP_Purified_MAP6_Anitbody_3_123118.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-MAP6 (STOP) antibody (clone STOP). Lane 1: Molecular weight marker; Lane 2: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-MAP6 (STOP) antibody (clone STOP) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-MAP6 (STOP) antibody (clone STOP) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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824701 | 100 µL | 275 CHF |
Microtubule-associated protein 6 (MAP6), also known as stable tubule-only polypeptide or STOP, is a calmodulin-binding protein that is involved in microtubule stabilization and is also regulated by calmodulin. MAP6 regulates lysosomal trafficking and is involved in dendrite maintenance and morphogenesis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against the STOP protein derived from the brains of rats.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
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WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 5 µg per ml. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).
- RRID
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AB_2564883 (BioLegend Cat. No. 824701)
Antigen Details
- Structure
- Expected MW: 145 kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 4135 View all products for this Gene ID
- UniProt
- View information about MAP6 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All MAP6 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-MAP6 (STOP) | STOP | WB,IHC-P |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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