- Clone
- OX-114 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Basigin
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD147 (clone OX-114) purified overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective. -
C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mouse CD147 (clone OX-114) purified overnight at 4°C, followed by 2.5 µg/ml of anti-mouse IgG (clone Poly4053) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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123701 | 50 µg | 65 CHF |
CD147 is a widely expressed membrane glycoprotein and reported to be implicated in cell adhesion, embryonic development, T cell activation and many other functions. It is expressed in thymocytes and many other cells, including epithelial and endothelial cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone OX-114 has been shown to bind both two and three domain forms of mouse CD1471.
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Application References
(PubMed link indicates BioLegend citation) -
- Hanna SM, et al. 2003. BMC Biochemistry 4:17.
- RRID
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AB_1227519 (BioLegend Cat. No. 123701)
Antigen Details
- Structure
- Single chain type I transmembrane 58 kd heavily glycosylated protein. N terminal extracellular part consists of two C2 type Ig-like domains which are highly glycosylated. Recently reported: isoform with an additional membrane-distal Ig-like domain.
- Distribution
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CD147 is expressed at varying levels in many cells types, including haematopoietic, epithelial, and endothelial cells. Expressed on immature thymocytes and at varying levels on spleen cells , bone marrow cells and macrophages.
- Function
- CD147 is implicated in multiple functions, such as matrix metalloproteinase induction, cell adhesion, retinal cell development, HIV attachment, embryonic development and T cell activation.
- Ligand/Receptor
- cyclophilin
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Hanna SM, et al. 2003. BMC Biochemistry 4:17
2. Yurchenko V, et al. 2005. Immunology 117:301
3. Renno T, et al. 2002. J. Immunol. 168:4946 - Gene ID
- 12215 View all products for this Gene ID
- UniProt
- View information about CD147 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD147 | OX-114 | FC,IHC-F,IP |
FITC anti-mouse CD147 | OX-114 | FC |
PE anti-mouse CD147 | OX-114 | FC |
Alexa Fluor® 594 anti-mouse CD147 | OX-114 | IHC-F |
Alexa Fluor® 647 anti-mouse CD147 | OX-114 | IHC-F |
Alexa Fluor® 488 anti-mouse CD147 | OX-114 | IHC-F,3D IHC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD147
C57BL/6 mouse frozen testis section was fixed with 4% parafo... C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
FITC anti-mouse CD147
C57BL/6 thymocytes stained with OX-114 FITC -
PE anti-mouse CD147
C57BL/6 thymocytes stained with OX-114 PE -
Alexa Fluor® 594 anti-mouse CD147
C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
Alexa Fluor® 647 anti-mouse CD147
C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
Alexa Fluor® 488 anti-mouse CD147
C57BL/6 mouse frozen testis section was fixed with 4% parafo... Dissected C57/B6 mouse testis was immersed in 4% paraformal... Formalin-fixed, 300 micron-thick mouse testis section was bl... Paraformaldehyde-fixed (4%), 500 µm-thick mouse testis secti...
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