Purified anti-mouse CD54 Antibody

Pricing & Availability
Clone
W21055C (See other available formats)
Regulatory Status
RUO
Other Names
ICAM-1, Ly-47
Isotype
Rat IgG2a, κ
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Product Citations
publications
W21055C_PURE_CD54_1_071723
C57BL/6 mouse splenocytes stained with purified anti-mouse CD54 (clone W21055C) (filled histogram), or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • W21055C_PURE_CD54_1_071723
    C57BL/6 mouse splenocytes stained with purified anti-mouse CD54 (clone W21055C) (filled histogram), or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • W21055C_PURE_CD54_2_071723
    IHC staining of anti-mouse CD54 (clone W21055C) on C57BL/6 mouse frozen small intestine. After fixing with 4% paraformaldehyde (PFA) for ten minutes, the tissue was incubated with 10 µg/mL of purified anti-mouse CD54 (clone W21055C) overnight at 4°C, followed by incubation with 2.5 µg/mL of anti-rat IgG Alexa Fluor® 594 (red) (Cat. No. 405422) for two hours at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
  • W21055C_PURE_CD54_3_071723
    IHC staining of anti-mouse CD54 (clone W21055C) on C57BL/6 mouse frozen brain tissue (negative control). After fixing with 4% paraformaldehyde (PFA) for ten minutes, the tissue was incubated with 10 µg/mL of purified anti-mouse CD54 (clone W21055C) overnight at 4°C, followed by incubation with 2.5 µg/mL of anti-rat IgG Alexa Fluor® 594 (red) (Cat. No. 405422) for two hours at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective.
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165902 100 µg 105 CHF
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Description

CD54, also known as ICAM-1 and Ly-47, is a 90 kD immunoglobulin superfamily member. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse ICAM-1 (28-485) with human Fc chimera plus transfectant (full length)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemal staining on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3097209 (BioLegend Cat. No. 165902)

Antigen Details

Structure
Ig superfamily, 90 kD
Distribution

Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Immune reaction, inflammation, adhesion
Ligand/Receptor
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Springer TA. 1990. Nature. 346:425-34.
  3. Rothlein R, et al. 1986. J Immunol. 137:1270.
Gene ID
15894 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.17.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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