Purified anti-mouse CD62E (E-selectin) Antibody

Pricing & Availability
Clone
W20055M (See other available formats)
Regulatory Status
RUO
Other Names
E-selectin, ELAM-1, LECAM-1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20055M_PURE_CD62E_Antibody_122823
bEnd.3 cells (mouse endothelial cells) were stained with purified anti-mouse CD62E (E-selection) (clone W20055M) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • W20055M_PURE_CD62E_Antibody_122823
    bEnd.3 cells (mouse endothelial cells) were stained with purified anti-mouse CD62E (E-selection) (clone W20055M) (filled histogram) or purified rat IgG2b, κ isotype control (open histogram) followed by anti-rat IgG PE.
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132402 100 µg 135 CHF
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Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I transmembrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells. CD62E is involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Sialyl Lewis X (CD15s) and PSGL-1 (CD162).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Transfectant cells (29-564 aa) and recombinant protein (29-564 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow cytometry testing showed that clone W20055M does not affect binding of clone W20055P on target cells.

RRID
AB_3097461 (BioLegend Cat. No. 132402)

Antigen Details

Structure
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution

Highly expressed on activated endothelial cells

Function
Involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor
Sialyl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
20339 View all products for this Gene ID
UniProt
View information about CD62E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.28.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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