- Clone
- A15105J (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15105J_PURE_MRP-14_Antibody_WB_010218 A15105J_PURE_MRP-14_Antibody_WB_010218](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15105J_PURE_MRP-14_Antibody_WB_010218.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Recombinant human S100A8 protein (Cat. No. 719902, lane 1), S100A9 protein( lane 2) and total lysates (15 µg protein) from HeLa (negative control, lane 3) , PBMC (lane 4) were resolved by 4-20% Tris-Glycine electrophoresis, transferred to nitrocellulose, and probed with 1:5000 (0.1 µg/mL) diluted purified anti-MRP-14 (S100A9) (clone A10105J). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: Molecular weight ladder.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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600301 | 25 µg | 101 CHF | ||||
600302 | 100 µg | 253 CHF |
MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and has been considered an endogenous activator of Toll-like receptor 4. S100A9 has been identified as a marker for CD14(+) HLA-DR(-/low) MDSCs.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human S100A8/S100A9 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.5 µg per mL (1:1000-1:10000). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone does not recognize S100A8. The 114 amino acid recombinant S100A9 protein has a predicted Mw of ~13 kD for monomer and ~26 kD for dimer.
- Product Citations
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- RRID
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AB_2721746 (BioLegend Cat. No. 600301)
AB_2721747 (BioLegend Cat. No. 600302)
Antigen Details
- Structure
- Belongs to S100 family of proteins containing 2-EF-hand calcium -binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8. S100A9 has 114 amino acids with the predicted Mw of ~13 kDa.
- Distribution
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MDSCs, neutrophils, monocyte, and ileal tissue eosinophils
- Function
- Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
- Interaction
- Calcium, S100A8
- Ligand/Receptor
- RAGE, TLR4
- Cell Type
- Neutrophils, Monocytes, Eosinophils
- Biology Area
- Cancer Biomarkers, Cell Biology, Immunology, Innate Immunity
- Antigen References
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1. Loser K, et al. 2010. Nat. Med. 16:713.
2. Cheng P, et al. 2008. J. Exp. Med. 205:2235.
3. Leukert N, et al. 2006. J. Mol. Biol. 359:961-72.
4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402.
5. Zhao F, et al. 2012. Immunology. 136:176. - Gene ID
- 6280 View all products for this Gene ID
- UniProt
- View information about MRP-14 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All MRP-14 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-MRP-14 (S100A9) | A15105J | WB |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-MRP-14 (S100A9)
Recombinant human S100A8 protein (Cat. No. 719902, lane 1), ...
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