- Clone
- W18295A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Phosphoglycerate Kinase 1 (PGK1), cell migration-inducing gene 10 protein, primer recognition protein 2 (PRP2), Epididymis Secretory Sperm Binding Protein Li 68p, EC 2.7.2.3
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W18295A_PURE_PGK1_Antibody_1_030822 W18295A_PURE_PGK1_Antibody_1_030822](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W18295A_PURE_PGK1_Antibody_1_030822.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-PGK1 (clone W18295A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker -
IHC staining of purified anti-PGK1 (clone W18295A) on formalin-fixed paraffin-embedded mouse eye tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1x (Cat. No. 928502), the tissue was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the product’s protocol. The image was captured with a 40X objective. Scale bar: 50 µm -
ICC staining of purified anti-PGK1 (clone W18295A) on HeLa cells. The cells were fixed and permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with either purified rat IgG2a, λ isotype control (Cat. No. 402301) (left panel) or purified anti-PGK1 (clone W18295A) (right panel) at 1.0 µg/mL overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) (Cat. No. 422801), and the image was captured with a 40X objective. Scale bar = 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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603251 | 25 µg | 118 CHF | ||||
603252 | 100 µg | 293 CHF |
Phosphoglycerate kinase 1 (PGK1) is one of two enzymes, along with its isozyme PGK2, which catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, and transfers the phosphate group to ADP to produce ATP. This step is notable as the “break even” point of glycolysis where ATP expenditure matches ATP output. Additionally, PGK1 functions as a protein kinase, and is also involved in transcription initiation as a cofactor for DNA polymerase alpha. The gene encoding PGK1 is on the X chromosome, and mutations in PGK1 are associated with various cancers, and X-linked recessive variants of anemia, mental disorders, and myopathy.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial fragment of recombinant Human PGK1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2910481 (BioLegend Cat. No. 603251)
AB_2910481 (BioLegend Cat. No. 603252)
Antigen Details
- Structure
- PGK1 is a 417 amino acid protein with a predicted molecular weight of 44.6 kD.
- Distribution
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PGK1 is widely expressed in the cytoplasm of most tissues.
- Function
- Enzyme catalyzing the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Protein kinase.
- Interaction
- PDHK1
- Biology Area
- Cancer Biomarkers
- Molecular Family
- Enzymes and Regulators, Protein Kinases/Phosphatase
- Antigen References
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- Fu Q and Yu Z. 2020. Life Sci. 256:117863.
- Gene ID
- 5230 View all products for this Gene ID
- UniProt
- View information about PGK1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All PGK1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PGK1 | W18295A | WB,IHC-P,ICC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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