- Clone
- W23013A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PLCG1, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1, phospholipase C, gamma 1 (PLCG1), phospholipase C-II (PLC-II)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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W23013A_PURE_PLCg1_WB_052824](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W23013A_PURE_PLCg1_WB_052824.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein per lane) from indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-PLCγ1 (clone W23013A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker -
IHC staining of Purified anti-PLCγ1 (clone W23013A) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) Purified anti-PLCγ1 (clone W23013A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
638051 | 25 µg | 175 CHF | ||||
638052 | 100 µg | 430 CHF |
Phospholipase C gamma 1 (PLCγ1) is a signaling enzyme involved in intracellular signal transduction. In response to activation of receptor-type tyrosine kinases, phospholipase C (PLC) enzymes cleave the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) into second messengers, namely inositol 1,4,5-triposphate (IP3) and diacylglycerol (DAG). IP3 causes the release of calcium from the endoplasmic reticulum to begin an intracellular signaling cascade, while DAG activates Protein Kinase C.
There are four families of PLCs. The PLCγ family, consisting of PLCγ1 and PLCγ2 in humans, uniquely contains SRC domains SH2 and SH3. Receptor tyrosine kinases interact with these SRC domains to enzymatically activate PLCγ1 via phosphorylation. PLCγ1 is essential for cell proliferation, differentiation, and motility. Overexpression or activation of PLCγ1 has been implicated in breast and prostate cancers as well as cancer cell invasion.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of the C-terminus of human PLCγ1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody (clone W23013A) does not cross-react with mouse PLCγ1 in Western Blot.
This antibody (clone W23013A) is not suitable for either immunocytochemistry (ICC) or intracellular flow cytometry (ICFC) due to dim signal.
For immunohistochemistry (IHC), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) is not recommended due to non-specific staining.
Antigen Details
- Structure
- PLCγ1 is a 1290 amino acid protein with a predicted molecular weight of 148.5 kD.
- Distribution
-
Cytoplasmic
- Function
- Enzyme
- Interaction
- GRB2, and various Receptor tyrosine kinases (RTKs) such as PDGFRA, PDGFRB, EGFR, RET, FLT1, TRKA, BLNK, etc.
- Cell Type
- T cells
- Biology Area
- Cell Proliferation and Viability, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Ji QS, et al. 1997. Proc Natl Acad Sci. 94:2999-3003.
- Jang HJ, et al. 2018. Adv Biol Regul. 67:179-189.
- Yang YR, et al. 2016. Adv Biol Regul. 61:80-5.
- Gene ID
- 5335 View all products for this Gene ID
- UniProt
- View information about PLCgamma1 on UniProt.org
Related FAQs
Other Formats
View All PLCγ1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PLCγ1 | W23013A | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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