- Clone
- W16098A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prospero homeobox protein 1, Homeobox prospero-like protein PROX1,
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W16098A_PURE_Prox1_Antibody_051017 W16098A_PURE_Prox1_Antibody_051017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W16098A_PURE_Prox1_Antibody_051017.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg protein) from HeLa (negative control), HepG2 and NIH3T3 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-Prox1 (clone W16098A) antibody (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti-Prox1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH antibody. Lane M: molecular weight ladder. -
HepG2 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.5 µg/ml anti- Prox1 antibody (clone W16098A) overnight at 4° followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Actin filaments were labeled with Flash Phalloidin™ Green 488. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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696902 | 100 µg | 282 CHF |
Prospero homeobox protein 1 (PROX1) is a homeobox transcription factor responsible for progenitor cell differentiation and the development of several organs, such as the central nervous system, lymphatics, pancreas, heart, lens, skeletal muscle, and liver. Prox1-deficient mice lack lymphatic systems and die at day 14.5 of embryogenesis due to multiple developmental defects. In addition to its role in physiological development, PROX1 is involved in carcinogenesis and can act either as a tumor suppressor or as an oncogene depending on the tissue and its expression level. Prox1 is also implicated in the regulation of metabolism in the liver by regulating activities of nuclear receptors including hepatocyte nuclear factor 4a (HNF4a) and liver receptor homolog-1 (LRH-1).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human Prox1 recombinant protein (2-259 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 5 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone does not cross react with mouse (in-house tested).
- RRID
-
AB_2687107 (BioLegend Cat. No. 696902)
Antigen Details
- Structure
- 737 amino acids with a predicted molecular weight of 83 kD. Contains a C-terminal Prospero-type homeo domain responsible for DNA binding.
- Distribution
-
Nucleus.
- Function
- PROX-1 is a homeobox transcription factor involved in the embryonic development and carcinogenesis.
- Interaction
- Interacts with RORA, RORC, and HNF4a.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Kivelä R, et al. 2016. Nat. Commun. 7:13124.
2. Stergiopoulos A, et al. 2015. Front Cell Neurosci. 8:454.
3. Ragusa S, et al. 2014. Cell Rep. 8:1957.
4. Elsir T, et al. 2012. Cancer Metastasis Rev. 31:793.
5. Karalay O, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:5807.
6. Johnson NC, et al. 2008. Genes Dev. 22:3282. - Gene ID
- 5629 View all products for this Gene ID
- UniProt
- View information about Prox1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Prox1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Prox1 | W16098A | WB,ICC |
Alexa Fluor® 594 anti-Prox1 | W16098A | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Prox1
Total cell lysates (15 µg protein) from HeLa (negative contr... HepG2 cells were fixed with 4% paraformaldehyde (PFA) for 1... -
Alexa Fluor® 594 anti-Prox1
HepG2 cells were fixed with 4% paraformaldehyde (PFA) for 10...
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