- Clone
- O91F3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PSMA6, proteasome subunit alpha type-6, PSA6, 27 kDa prosomal protein, PROS-27, p27K, multicatalytic endopeptidase complex iota chain, proteasome iota chain
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![O91F3_PURE_PSMA6_Antibody_081219 O91F3_PURE_PSMA6_Antibody_081219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/O91F3_PURE_PSMA6_Antibody_081219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from the indicated cell lines, HEK-293, HeLa, BeWo, HepG2, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-PSMA6 antibody, clone O91F3, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
670601 | 25 µg | 101 CHF | ||||
670602 | 100 µg | 253 CHF |
Proteasome subunit-α type-6 protein, or PSMA6, is an essential subunit of the 20S catalytic core proteasome complex. As a component of the complex, it plays a central role in the proteolytic degradation of ubiquitinated proteins, thereby, maintaining protein homeostasis by removing misfolded, damaged, or excess proteins. It is involved in multiple pathways including spermatogenesis and peptide processing for MHC class I binding, and has been proposed as a therapeutic target for lung cancer.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length recombinant human PSMA6 protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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O91F3 failed ICC testing; HeLa cells were fixed with 4% paraformaldehyde and tested using two permeabilization methods (Methanol and Triton X-100). The clone failed ICC testing due to non-specific staining of cytosol.
IP has not been tested on clone O91F3.
- RRID
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AB_2814522 (BioLegend Cat. No. 670601)
AB_2814523 (BioLegend Cat. No. 670602)
Antigen Details
- Structure
- PSMA6 is a 246 amino acid protein with a predicted molecular weight of 27 kD.
- Distribution
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Nucleus/Ubiquitously expressed
- Function
- Protein homeostasis, proteostasis
- Biology Area
- Ubiquitin/Protein Degradation
- Antigen References
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- Li Z, et al. 2010. Biochem. Cell. Biol. 88:635-48.
- Wenzel T, Baumeister W. 1995. Nat. Struct. Biol. 2:199-204.
- Gene ID
- 5687 View all products for this Gene ID
- UniProt
- View information about PSMA6 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All PSMA6 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PSMA6 | O91F3 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-PSMA6
Whole cell extracts (15 µg protein) from the indicated cell ...
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