Purified anti-rat CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
10D6 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10D6_PURE_CD326EpCAM_Antibody_083120.png
Rat frozen kidney section was fixed with 4% paraformaldehyde for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL purified CD326 (clone 10D6) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor™ 594 anti-mouse IgG (Poly4053) (red) for one hour at room temperature. The image was captured by 10X objective.
  • 10D6_PURE_CD326EpCAM_Antibody_083120.png
    Rat frozen kidney section was fixed with 4% paraformaldehyde for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL purified CD326 (clone 10D6) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor™ 594 anti-mouse IgG (Poly4053) (red) for one hour at room temperature. The image was captured by 10X objective.
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251002 100 µg 288 CHF
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Description

Ep-CAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of Ep-CAM seems to correlate inversely with the level of E-cadherin (CD324). Ep-CAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2876582 (BioLegend Cat. No. 251002)

Antigen Details

Function
Mediates calcium-independent homophilic cell-cell adhesion
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Gene ID
171577 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org
Go To Top Version: 1    Revision Date: 08.31.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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