- Clone
- A17123A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mitochondrial import receptor subunit TOM40 homolog
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A17123A_PURE_TOMM40_Antibody_07202018 A17123A_PURE_TOMM40_Antibody_07202018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A17123A_PURE_TOMM40_Antibody_07202018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-TOMM40 antibody (clone A17123A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.001 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The band at ~60 kD is potentially the TOM/TIM complex.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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853601 | 25 µg | 106 CHF | ||||
853602 | 100 µg | 276 CHF |
Mitochondria fulfill central functions in cellular energetics, metabolism, and signaling. The outer membrane translocator complex (the TOM complex) imports most mitochondrial proteins, and TOMM40 is the key subunit of the complex, the pore through which the vast majority of imported proteins must pass. TOMM40 possesses chaperone-like properties, which helps expedite movement of the pre-proteins through the channel. TOM cooperates with the TIM23 (Translocase of the Inner Membrane 23) complexes to import matrix-targeted proteins. TOMM40 is implicated in many neurodegenerative diseases; reduced TOMM40 mRNA expression in Alzheimer’s disease patients was shown to correlate with cognitive decline.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A synthetic peptide corresponding to TOMM40 protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This antibody shows negligible cross-reactivity with human TOMM40.
- RRID
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AB_2749940 (BioLegend Cat. No. 853601)
AB_2749941 (BioLegend Cat. No. 853602)
Antigen Details
- Structure
- TOMM40 is a 361 amino acid protein with a molecular weight mass of ~40 kD.
- Distribution
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Tissue distribution: TOMM40 is expressed in numerous tissues including the central nervous system (CNS), muscle, and the immune systems.
Cellular distribution: Cytosolic, mitochondria, and nucleus. - Function
- TOMM40 is the key subunit of the TOM complex, which imports proteins into the mitochondria.
- Interaction
- TOMM40 Interacts with mitochondrial targeting sequences and the TIM complex.
- Biology Area
- Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors
- Molecular Family
- Mitochondrial Markers
- Antigen References
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- Gottschalk WK, et al. 2014. J. Parkinsons Dis. Alzheimers Dis. 12.
- Shiota T, et al. 2015. Science 349:1544-8.
- Mise A, et al. 2017. J. Alzheimers Dis. 60:1107-1117.
- Ahting U, et al. 2001. J. Cell. Biol. 153:1151-60.
- Gene ID
- 10452 View all products for this Gene ID
- UniProt
- View information about TOMM40 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All TOMM40 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TOMM40 | A17123A | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-TOMM40
Western blot of purified anti-TOMM40 antibody (clone A17123A...
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