- Clone
- A18140A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, Ubiquitin carrier protein O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kD, Ubiquitin-protein ligase O
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A18140A_PURE_UBE20_Antibody_1_120219 A18140A_PURE_UBE20_Antibody_1_120219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A18140A_PURE_UBE20_Antibody_1_120219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-UBE2O antibody (clone A18140A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
ICC staining of purified anti-UBE2O antibody (clone A18140A) on A431 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
870201 | 25 µg | 106 CHF | ||||
870202 | 100 µg | 276 CHF |
Ubiquitin-conjugating enzyme E2 O (UBE2O) is an E2/E3 hybrid enzyme that displays both E2 and E3 ligases activities and mediates ubiquitination of many target proteins involved in a wide range of processes, including the 5’-AMP-activated protein kinase catalytic subunit a2 (AMPKa2), tumor suppressor ubiquitin carboxyl-terminal hydrolase BAP1, mixed-lineage leukemia (MLL) protein, SMAD family member 6 (SMAD6), transcription factor c-Maf, and free ribosomal proteins. Abnormal expression and mutations in UBE2O are observed in tumorigenesis and are associated with poor prognosis of gastric, lung, breast, and prostate cancers.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human UBE2O protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.2 - 5.0 µg/mL. For immunocytochemistry, a concentration range of 0.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2820188 (BioLegend Cat. No. 870201)
AB_2820189 (BioLegend Cat. No. 870202)
Antigen Details
- Structure
- Human UBE2O is a 1,292 amino acid protein with a predicted molecular mass of 141 kD and observed molecular mass of ~200 kD
- Distribution
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Tissue distribution: Brain, skeletal muscle, heart
Cellular distribution: Nucleus, cytosol - Function
- UBE2O is an E2/E3 hybrid ubiquitin-protein ligase that is crucial for protein ubiquitination and degradation.
- Cell Type
- Astrocytes, Microglia, Neurons, Oligodendrocytes
- Biology Area
- Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Ubiquitin/Protein Degradation
- Molecular Family
- Ligases
- Antigen References
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- Ullah K, et al. 2019. FEBS J. 286(11):2018-2034.
- Chen S, et al. 2018. J Biol Chem. 293(29):11296-11309.
- Mashtalir N, et al. 2014. Mol Cell. 54(3):392-406.
- Gene ID
- 63893 View all products for this Gene ID
- UniProt
- View information about UBE2O on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All UBE2O Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-UBE2O | A18140A | WB,ICC |
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