Loading...

Purified Non-muscle Myosin Heavy Chain II-B Antibody (Previously Covance catalog# PRB-445P)

Pricing & Availability
Clone
Poly19099 (See other available formats)
Regulatory Status
RUO
Other Names
Myosin-10, NMMHC-B, myosin heavy chain 10, nonmuscle myosin heavy chain-B, nonmuscle myosin heavy chain IIB, cellular myosin heavy chain, type B type B
Previously
Covance Catalog# PRB-445P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
Poly19099_Pure_ChainII-B_Antibody_1_111417
Western blot analysis of cell lysates from Hela (positive control), Daudi (low expression negative control) (Human) and UMR106 (Rat) using Non-muscle Myosin Heavy Chain II-B (Myosin-10) Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • Poly19099_Pure_ChainII-B_Antibody_1_111417
    Western blot analysis of cell lysates from Hela (positive control), Daudi (low expression negative control) (Human) and UMR106 (Rat) using Non-muscle Myosin Heavy Chain II-B (Myosin-10) Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
  • Poly19099_Pure_ChainII-B_Antibody_2_111417
    Immunofluorescence of HeLa cells with (A) Rabbit Isotype control (Cat. No. 910801) or (B-D) Non-muscle Myosin Heavy Chain II-B primary Antibody. Alexa Fluor® 594 (Red) Donkey anti-Rabbit IgG (Cat. No. 406418) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A) is 1/30, and (B-D) is 1/60. Concentrations for (A, B) is 2 µg/ml, (C) is 1 µg/ml, (D) is 0.5 µg/ml.
Cat # Size Price Quantity Check Availability Save
909902 25 µL 89 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
909901 100 µL 288 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Non-muscle Myosin Heavy Chain II-B is a member of the myosin superfamily. There are three isoforms of myosin II, called myosin IIA, myosin IIB and myosin IIC. These proteins are involved in cell motility, maintenance of cell shape, and cytokinesis. Each type of myosin II protein consists of two heavy chains and four light chains. The heavy chains each have two parts: a myosin head-like domain and a tail region. The head region interacts with actin, a protein that is important for cell movement and shape. The long tail region interacts with other proteins, including the tail regions of other myosin proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against the peptide SDVNETQPPQSE corresponding to the C-terminus of nonmuscle myosin heavy chain isoform B (MHC-B).
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Verified
IHC-P, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:1000 - 1:2000). For immunocytochemistry, the suggested use of this reagent is 0.5 - 2.0 µg per ml (1:500 - 1:2000). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC) and immunofluorescence (IF).

*Predicted MW = 200 kD

Positive Control: human smooth muscle


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Ma X, et al. 2007. Mol Biol Cell. 18:2305. (IF)
  2. Golomb E, et al. 2004. J Biol Chem. 279:2800. (WB, IF)
  3. Song J, et al. 2001. Mol Cell Neurosci. 17:624.
  4. Kelley CA, et al. 1996. J Cell Biol. 134:675.
  5. Kelley CA, et al. 1995. J Biol Chem. 270:1395.
  6. Phillips CL, et al. 1995. J Muscl Res Cell Motil. 16:379.
  7. Skau CT, et al. 2015. PNAS. 112:2447. PubMed
  8. Wang H, et al. 2008. J. Cell Biol. 182: 1171. (ICC, WB) PubMed
  9. Hirota Y, et al. 2010. Development. 137: 3037. (IF) PubMed
Product Citations
  1. Chopra A, et al. 2018. Dev Cell. 44:87. PubMed
  2. Wang XW, et al. 2020. Cell Rep. 31:107537. PubMed
  3. Priya R, et al. 2017. Mol Biol Cell. 28(1):12-20. PubMed
  4. Tokuda S, Hirai T, and Furuse M. 2017. PLoS One.. 10.1371/journal.pone.0182521. PubMed
  5. Li X, et al. 2015. Proc Natl Acad Sci U S A. 112:6080. PubMed
  6. Aguilar-Cuenca R, et al. 2020. Current Biology. 30(13):2446-2458.e6. PubMed
  7. Belardi B, et al. 2020. Developmental Cell. 54(6):792-804.e7. PubMed
  8. Cartagena-Rivera A, Van Itallie C, and Anderson J. 2017. Nat Commun. . 10.1038/s41467-017-01145-8. PubMed
  9. Bonneaud TL, et al. 2022. Cell Death Dis. 13:787. PubMed
  10. Otterpohl K, et al. 2017. Physiol Rep.. 10.14814/phy2.13513. PubMed
  11. Sumigray KD et al. 2018. Developmental cell. 45(2):183-197 . PubMed
  12. Martínez-Ara G, et al. 2022. Nat Commun. 13:5400. PubMed
  13. S Tokuda, T Hirai, M Furuse 2016. PLoS One. 11:e0166904. PubMed
  14. Jiu Y, et al. 2019. Curr Biol. 29:81. PubMed
  15. Eaton AF, et al. 2019. Mol Biol Cell. 30:2037. PubMed
  16. Yu-Kemp HC, et al. 2022. J Cell Biol. 221:. PubMed
  17. Weienbruch K, et al. 2021. eLife. 0.416666666666667. PubMed
  18. Salomon J, et al. 2017. Nat Commun. 8:13998. PubMed
  19. Hirota Y, et al. 2010. Development. 137:3037-3046. PubMed
  20. Ninomiya K, et al. 2021. J Cell Sci. 134:. PubMed
  21. Zhou R, et al. 2022. Nat Commun. 13:3196. PubMed
  22. Butler MB, et al. 2019. J Cell Sci. 132. PubMed
  23. Ninomiya K, et al. 2020. J Cell Sci. . PubMed
  24. Zhovmer AS, et al. 2019. Mol Biol Cell. 30:1961. PubMed
  25. Naydenov NG, et al. 2022. Cells. 11:. PubMed
  26. Skau C, et al. 2015. Proc Natl Acad Sci U S A. 112: 2447 - 2456. PubMed
  27. Trivedi N, et al. 2017. Nat Commun. 8:14484. PubMed
  28. Ramkumar N, et al. 2016. Nat Cell Biol. 18:1281-1291. PubMed
  29. Awadia S, et al. 2019. J Cell Biol. 218:2699. PubMed
  30. Zaman R, et al. 2021. J Cell Biol. 220:. PubMed
  31. Wang H, et al. 2008. J Cell Biol. 182:1171-1184. PubMed
  32. Van Itallie CM, et al. 2018. J Cell Sci. 131:. PubMed
  33. Maniou E, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  34. Loebel D, et al. 2016. Biol Open. 10.1242/bio.014415. PubMed
  35. Ning W, et al. 2020. Cell Stem Cell. 28(3):436-452.e5. PubMed
  36. Yamada S, et al. 2019. J Dent Res. 98:1253. PubMed
  37. Fenix AM, et al. 2018. Elife. 7:. PubMed
  38. Nguyen-Ngoc K, et al. 2017. J Cell Sci. 10.1242/jcs.208546. PubMed
RRID
AB_2749903 (BioLegend Cat. No. 909902)
AB_2565101 (BioLegend Cat. No. 909901)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Gene ID
4628 View all products for this Gene ID
UniProt
View information about Myosin-10 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 9    Revision Date: 08.12.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account