- Regulatory Status
- RUO
- Other Names
- Obese Protein, Obesity Factor, LEPD, OB, OBS
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
444309 | 1 Pre-coated Plate | 595 CHF |
Human Leptin is a 167 amino acid protein and approximately 16 kD in mass. Leptin is encoded by the obese (ob) gene and the gene locates on chromosome 7. Leptin is an adipocyte-derived hormone and mainly secreted by adipocyte; small amounts of Leptin are expressed by the cells in the epithelium of the stomach and in the placenta. Leptin’s effects on body weight are mediated through impacts on hypothalamic center that control feeding behavior, hunger, body temperature, and energy expenditure. Leptin importantly regulates food intake, body weight, metabolism, energy expenditure, reproductive function, and adiposity through hypothalamic Leptin receptor. Leptin is also the member of long-chain helical cytokine family.
The BioLegend RAPID MAX™ Human Leptin ELISA Kit is a one step, one wash, 70-minute enzyme-linked immunosorbent sandwich assay (ELISA) with a pre-coated, 96-well strip plate that immobilizes the biotinylated capture antibody. Standards and samples are added into the wells, followed by an antibody cocktail. The antibody cocktail consists of a biotinylated monoclonal capture antibody and an HRP-conjugated monoclonal detection antibody. This kit is specifically designed for the accurate quantitation of human Leptin cell culture supernatant, serum, plasma. This kit is analytically validated with ready-to-use reagents.
Kit Contents
- Kit Contents
-
- RAPID MAX™ Pre-coated 96-well Strip Microplate
- Human Leptin Capture Antibody Concentrate (200X)
- Human Leptin Detection Antibody Concentrate (200X)
- Human Leptin Standard
- Assay Diluent B (5X), 12 mL
- Substrate Solution F
- Wash Buffer (20X)
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 19.6 ± 2.4 pg/mL
- Standard Range
- 62.5 - 4000 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
Antigen Details
- Cell Type
- Endothelial cells, Fibroblasts
- Biology Area
- Angiogenesis, Cell Biology, Signal Transduction
- Molecular Family
- Enzymes and Regulators
- Gene ID
- NA
- UniProt
- View information about Leptin on UniProt.org
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