Recombinant Mouse IL-21 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin-21
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Product Citations
publications
Mouse_IL-21_CF_RECOM_1_113023
Recombinant mouse IL-21 induces proliferation of CTLL-2 cells in a dose-dependent manner. The ED50 for this effect is 10 – 50 ng/mL.
  • Mouse_IL-21_CF_RECOM_1_113023
    Recombinant mouse IL-21 induces proliferation of CTLL-2 cells in a dose-dependent manner. The ED50 for this effect is 10 – 50 ng/mL.
  • Mouse_IL-21_CF_RECOM_2_113023
    Recombinant mouse IL-21 induces proliferation of HT-2 cells in a dose-dependent manner. The ED50 for this effect is 2.5 – 12.5 ng/mL.
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574508 500 µg 3275 CHF
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574506 100 µg 1031 CHF
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574502 10 µg 218 CHF
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574504 25 µg 411 CHF
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Description

IL-21 was identified by a functional screening from conditioned medium of activated T cells using Baf3-IL-21R transfectans. In normal B cells, IL-21 can mediate cell proliferation, growth arrest, and terminal differentiation, or apoptosis, depending on their activation status. IL-21 enhances B cell proliferation following incubation with an activating CD40 antibody. This result suggests that IL-21 enhances B cell function following T:B cell interactions. Nevertheless, IL-21 inhibits the proliferation of murine and human B cells stimulated with anti-IgM antibodies. In addition, IL-21 enhaces proliferation, cytotoxic activity, and IFNγ production by CD8-effector T cells, and induces terminal differentiation of activated natural killer (NK) cells. Also, IL-21 can drive Th17 responses in conjunction with TGFβ. Nevertheless, IL-21 is not essential for the differentiation of Th17 cells in vitro and in vivo, as it was showed using IL-21 and IL-21R-deficient mice. IL-6 induces the production of IL-21 from CD4 T cells upon TCR stimulation, and IL-6 and IL-21 are key players in the Tfh differentiation, characterized by increased protein expression of both Bcl6 and CXCR5. IL-21R complex is formed with the IL-21R-alpha chain and the common subunits (γc) shared with other interleukins such as IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21R is wildly expressed on T cells, B cells, NK cells, and dendritic cells in all lymphoid tissues, as well as some nonimmune cells, such as fibroblasts and epithelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse IL-21, amino acids Pro25-Ser146 (Accession# NP_068554) was expressed in E. coli.
Molecular Mass
The 122 amino acid recombinant protein has a predicted molecular mass of approximately 14.2 kD. The DTT-reduced and non-reduced proteins migrate at approximately 20 kD by SDS-PAGE. The N-terminal amino acid is Pro.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant mouse IL-21 induces the proliferation of mouse cytotoxic CTLL2 cells in a dose-dependent manner. The ED50 for this effect is 15 – 60 ng/mL. Mouse IL-21 induces proliferation of mouse lymphocytes HT-2 cells in a dose-dependent manner. The ED50 for this effect is 2.5 – 12.5 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Li C, et al. 2019. Immunity. 491:51. PubMed
  2. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed

Antigen Details

Structure
Interleukin
Distribution

CD4 activated T cells, Th17 cells, NK T cells.

Function
IL-21 induces T and B cell proliferation, B cell Ig class switching to IgG production, decreases DC function, enhances differentiation of effector and central memory T cells, and regulates T cell and hematopoietic progenitor cell homeostasis.
Interaction
T cells, B cells, NK cells, NKT cells, dendritic cells, fibroblast, epithelial cells.
Ligand/Receptor
IL-21R and the common γc subunit (CD132) are components of the IL-21R complex.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Parrish-Novak J, et al. 2000. Nature 408:57.
2. De Totero D, et al. 2006. Blood 107:3708.
3. Korn T, et al. 2007. Nature 448:484
4. Coquet JM, et al. 2008. J. Immunol. 180:7097.
5. Rincon M, et al. 2008. J. Exp. Med. 2.06:69.
6. Kaplan MH, et al. 2011. Blood 117:6198.
7. Eto D, et al. 2011. PLOS One 6(3):e17739.

Gene ID
60505 View all products for this Gene ID
UniProt
View information about IL-21 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 4    Revision Date: 11.30.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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