- Clone
- QA17A18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S152, T14, TNFRSF7
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA17A18_SPKYG581_CD27_Antibody_121720.png QA17A18_SPKYG581_CD27_Antibody_121720.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA17A18_SPKYG581_CD27_Antibody_121720.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD3 APC and anti-human CD27 recombinant (clone QA17A18) Spark YG™ 581 (left) or with anti-human CD3 APC only (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
393215 | 25 tests | 124 CHF | ||||
393216 | 100 tests | 265 CHF |
CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm. - Excitation Laser
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Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Clone QA17A18 does not block clones 0323, M-T271, or LG.3A10, indicating a unique epitope.
- RRID
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AB_2890830 (BioLegend Cat. No. 393215)
AB_2890830 (BioLegend Cat. No. 393216)
Antigen Details
- Structure
- TNF-R superfamily, type I transmembrane glycoprotein.
- Distribution
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Medullary thymocytes, T and B cell subsets, NK cells.
- Ligand/Receptor
- CD70
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
- Knapp W, et al. 1989. Immunol. Today 10:253-8
- Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
- Hintzen R, et al. 1994. Immunol. Today 15:307.
- Agematsu K, et al. 1995. J. Immunol. 154:3627.
- Gene ID
- 939 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
Related FAQs
Other Formats
View All CD27 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD27 Recombinant Antibody | QA17A18 | FC |
Alexa Fluor® 647 anti-human CD27 Recombinant Antibody | QA17A18 | FC |
PerCP/Cyanine5.5 anti-human CD27 Recombinant Antibody | QA17A18 | FC |
PE/Cyanine7 anti-human CD27 Recombinant Antibody | QA17A18 | FC |
Alexa Fluor® 488 anti-human CD27 Recombinant Antibody | QA17A18 | FC |
PE anti-human CD27 Recombinant Antibody | QA17A18 | FC |
APC/Fire™ 810 anti-human CD27 Recombinant Antibody | QA17A18 | FC |
Spark YG™ 581 anti-human CD27 Recombinant Antibody | QA17A18 | FC |
Spark Red™ 718 anti-human CD27 Recombinant Antibody | QA17A18 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Alexa Fluor® 647 anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PerCP/Cyanine5.5 anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with True-St... -
PE/Cyanine7 anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with True-St... -
Alexa Fluor® 488 anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD3 Ale... -
PE anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD3 APC... -
APC/Fire™ 810 anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Spark YG™ 581 anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD27 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...
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